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NIR-II Nanoprobes: A Review of Components-Based Approaches to Next-Generation Bioimaging Probes
Bryce Dunn1, Marzieh Hanafi1, John Hummel2
1Department of Bioengineering, George Mason University, Fairfax, VA 22030, USA.
Bioengineering (Basel, Switzerland)
|August 26, 2023
Summary
Developing novel near-infrared fluorescent and photoacoustic contrast agents enhances deep tissue imaging. This review explores advanced nanoprobes for improved cellular and animal bioimaging applications.
Area of Science:
- Biomedical Imaging
- Nanotechnology
- Optical Imaging
Background:
- Optical imaging techniques like fluorescence and photoacoustic imaging provide cellular and tissue insights but are limited by poor depth penetration due to scattering and absorption.
- Near-infrared (NIR) window imaging enhances penetration by minimizing autofluorescence and scattering, crucial for deeper tissue visualization.
- Existing contrast agents for NIR imaging suffer from issues with photostability and specific targeting, necessitating the development of improved probes.
Purpose of the Study:
- To review recent advancements in near-infrared fluorescent and photoacoustic contrast agents.
- To explore encapsulation and functionalization technologies for creating targeted nanoscale imaging probes.
- To discuss the applications of these advanced probes in various bioimaging scenarios.
Main Methods:
- Comprehensive review of existing literature on NIR fluorescent and photoacoustic contrast agents.
- Analysis of organic dyes, polymers, and metallic nanostructures for their optical properties and imaging capabilities.
- Examination of encapsulation and functionalization strategies for probe development.
Main Results:
- NIR imaging agents, including organic dyes, polymers, and nanostructures, show promise for improved imaging depth and specificity.
- Encapsulation and functionalization techniques enable the creation of targeted, nanoscale probes for specific bioimaging tasks.
- These advanced probes are applicable in angiography, tumor imaging, and cell tracking.
Conclusions:
- Recent progress in NIR fluorescent and photoacoustic nanoprobes offers significant potential for enhanced preclinical diagnostic approaches.
- This review serves as a resource for researchers in biomedical imaging and nanotechnology to develop innovative imaging solutions.
- The development of targeted, stable, and effective NIR nanoprobes is critical for advancing diagnostic capabilities in healthcare.
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