Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ocular delivery of different valosin-containing protein (VCP) inhibitory formulations prevents retinal degeneration in rho<sup>∆I255</sup> mice.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

PARP Activity Is Essential for Retinal Photoreceptor Survival in the Human Homologous Rho<sup>I255del</sup> Mouse Model for Autosomal Dominant Retinitis Pigmentosa.

Journal of neurochemistry·2025
Same author

Quantitative Proteomics Identifies Potential Molecular Adaptations in Mouse Models of Congenital Stationary Night Blindness Type 2.

Molecular & cellular proteomics : MCP·2025
Same author

APOSTEL-R Recommendations for Reporting Retinal Optical Coherence Tomography Studies in Rodents.

Neurology(R) neuroimmunology & neuroinflammation·2025
Same author

Exploration of the visual streak of the Mongolian gerbil as a model for the human central retina.

Frontiers in medicine·2025
Same author

Intravital Imaging of Disease Mechanisms in a Mouse Model of CCM Skin Lesions-Brief Report.

Arteriosclerosis, thrombosis, and vascular biology·2024

Related Experiment Video

Updated: Jul 18, 2025

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
12:28

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Published on: March 12, 2022

3.7K

Mural Serum Response Factor (SRF) Deficiency Provides Insights into Retinal Vascular Functionality and Development.

Alexander Günter1, Vithiyanjali Sothilingam1, Michael M Orlich2

  • 1Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, University of Tübingen, 72076 Tübingen, Germany.

International Journal of Molecular Sciences
|August 26, 2023
PubMed
Summary

Serum response factor (SRF) deficiency in retinal mural cells causes vascular defects, including bulging and retinal detachment. These vascular changes lead to hypoxia and impaired retinal function.

Keywords:
mural cellsmurine disease modelsretinal imagingretinal ischemiaserum response factorvascular smooth-muscle cells

More Related Videos

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
07:32

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Published on: June 23, 2014

14.5K
Measuring Retinal Vessel Diameter from Mouse Fluorescent Angiography Images
04:04

Measuring Retinal Vessel Diameter from Mouse Fluorescent Angiography Images

Published on: May 19, 2023

685

Related Experiment Videos

Last Updated: Jul 18, 2025

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
12:28

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Published on: March 12, 2022

3.7K
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
07:32

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Published on: June 23, 2014

14.5K
Measuring Retinal Vessel Diameter from Mouse Fluorescent Angiography Images
04:04

Measuring Retinal Vessel Diameter from Mouse Fluorescent Angiography Images

Published on: May 19, 2023

685

Area of Science:

  • Vascular Biology
  • Ophthalmology
  • Genetics

Background:

  • Serum response factor (SRF) regulates muscle contraction and motility genes in vascular mural cells (MCs).
  • MC-SRF is crucial for retinal angiogenesis and vascular tone maintenance.
  • Understanding MC SRF's role in the mature retina is vital for vascular health.

Purpose of the Study:

  • To investigate the impact of MC-specific SRF deficiency on the mature retinal vasculature and function.
  • To analyze retinal morphology, vascular integrity, and function in Srf mutant mice.

Main Methods:

  • In vivo analysis using scanning laser ophthalmoscopy (SLO), angiography, and optical coherence tomography (OCT).
  • Functional assessment via full-field electroretinography (ERG).
  • Comparative analysis of retinal morphology and function in MC-SRF deficient mice.

Main Results:

  • MC SRF deficiency led to diverse vascular alterations, including bulging and arteriovenous (AV) anastomoses.
  • Severe cases presented with retinal detachment (RD) and irregular blood flow.
  • Hypoxia, indicated by negative ERG waveforms, resulted from disrupted retinal blood supply.
  • Significant interocular differences suggested a context-dependent, secondary nature of the observed defects.

Conclusions:

  • MC SRF deficiency severely impacts mature retinal vascular integrity and function.
  • The development of vascular pathologies appears to be secondary and influenced by local retinal environment.
  • Further research is needed to elucidate the downstream mechanisms and therapeutic targets.