Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman...

Area of Science:

• Cellular and Molecular Biology
• Spectroscopy and Imaging
• Cancer Research

Background:

• Multi-component drugs (MCDs) induce complex cellular changes at multiple levels.
• A non-invasive method is needed to study subcellular dynamic changes induced by MCDs.
• Gastric cancer therapy often involves MCDs, requiring detailed mechanistic understanding.

Purpose of the Study:

• To investigate the subcellular dynamic processes in gastric cancer cells treated with Compound Kushen Injection (CKI).
• To evaluate the utility of high-resolution confocal Raman spectroscopy (RS) for detecting drug-induced cellular variations.
• To establish a non-invasive method for screening MCDs in cancer therapy.

Main Methods:

• Utilized a homemade, high-resolution, confocal Raman spectroscopy (RS) device coupled with bright-field imaging.
• Collected simultaneous Raman spectra from the nucleus, cytoplasm, and intracellular vesicles of BGC823 gastric cancer cells.
• Treated cells with varying doses and times of Compound Kushen Injection (CKI) to observe dynamic changes.

Main Results:

• Raman spectroscopy revealed decreased DNA signatures in CKI-treated cells, consistent with known drug inhibition.
• Observed CKI-induced subcellular dynamic changes, including the formation of numerous intracellular vesicles.
• Detected deconstruction of cytoplasmic components and morphological alterations in treated cells.

Conclusions:

• High-resolution subcellular micro-Raman spectroscopy is a promising non-invasive technique for studying drug effects.
• The method can detect fine cellular dynamic variations induced by multi-component drugs.
• This approach has potential for the screening of MCDs in cancer therapy.