Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Probiotics01:22

Probiotics

Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
Introduction to the Human Microbiota01:22

Introduction to the Human Microbiota

Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity, and disease...
Microbiota Modulation by Antibiotics01:21

Microbiota Modulation by Antibiotics

Antibiotics have revolutionized modern medicine by saving countless lives from bacterial infections. However, their widespread use has inadvertently harmed the delicate balance of the human gut microbiota. The gut microbiota, a complex community of bacteria, archaea, viruses, and fungi, plays a vital role in regulating metabolism, immune responses, and maintaining intestinal health. Antibiotics, especially broad-spectrum types, disrupt this ecosystem by eradicating both harmful and beneficial...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fermented foods and inflammation: a crossover intervention trial with fresh and pasteurized sauerkraut.

European journal of clinical nutrition·2026
Same author

Enhancer adoption by an LTR retrotransposon generates viral-like particles, causing developmental limb phenotypes.

Nature genetics·2025
Same author

The impact of regular sauerkraut consumption on the human gut microbiota: a crossover intervention trial.

Microbiome·2025
Same author

Does Asthma Affect the Risk of Developing Breast Cancer?

Cancer medicine·2024
Same author

Human Gut Microbiota Plasticity throughout the Life Course.

International journal of environmental research and public health·2023
Same author

Assessment of the Gut Microbiota during Juice Fasting with and without Inulin Supplementation: A Feasibility Study in Healthy Volunteers.

Foods (Basel, Switzerland)·2022

Related Experiment Video

Updated: Jun 26, 2026

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
06:23

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Published on: February 15, 2019

14.3K

How Do Prebiotics Affect Human Intestinal Bacteria?-Assessment of Bacterial Growth with Inulin and XOS In Vitro.

Nelly Schropp1, Virginie Stanislas1, Karin B Michels1

  • 1Institute for Prevention and Cancer Epidemiology, Faculty of Medicine and Medical Center, University of Freiburg, 79110 Freiburg, Germany.

International Journal of Molecular Sciences
|August 26, 2023
PubMed
Summary

Prebiotics like inulin and xylooligosaccharides (XOS) show specific effects on gut bacteria growth. This study models bacterial responses to these prebiotics, revealing strain-specific preferences and new insights for synbiotic development.

Keywords:
XOScommensal bacteriacomplex carbon sourcegrowth curvegrowth modelinginulinmicrobiotaprebioticssaccharolytic fermentationxylooligosaccharides

More Related Videos

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
07:15

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

Published on: July 31, 2019

9.6K
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
00:06

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

7.1K

Related Experiment Videos

Last Updated: Jun 26, 2026

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
06:23

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Published on: February 15, 2019

14.3K
An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
07:15

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

Published on: July 31, 2019

9.6K
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
00:06

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

7.1K

Area of Science:

  • Microbiology
  • Gut Microbiome Research
  • Nutritional Science

Background:

  • Prebiotics selectively stimulate beneficial gut microorganisms, but their precise mechanisms are complex and host-dependent.
  • Understanding bacterial responses to prebiotics like inulin and xylooligosaccharides (XOS) is crucial for predicting health benefits.
  • Current knowledge lacks a comprehensive overview of which human gut bacteria are affected by specific prebiotics.

Purpose of the Study:

  • To develop a statistical model for comparing in vitro growth of diverse human gut bacteria on inulin and XOS.
  • To identify strain-specific bacterial preferences for inulin versus XOS.
  • To provide data for the rational design of novel synbiotics.

Main Methods:

  • Cultured typical human gut bacteria from various phylogenetic lineages in vitro.
  • Utilized continuous optical density (OD600) measurements to monitor bacterial growth.
  • Analyzed maximal growth rates (rmax), maximal optical density (ODmax), and area under the growth curve (AUC) for each strain and substrate.

Main Results:

  • Bacterial growth parameters (rmax, ODmax, AUC) demonstrated strain-specific utilization patterns for inulin and XOS.
  • Identified previously unrecognized bacterial growth on XOS, including specific strains of Streptococcus salivarius.
  • The model effectively compared growth dynamics across different bacterial lineages and prebiotic substrates.

Conclusions:

  • Prebiotic efficacy is highly strain-specific, necessitating detailed analysis beyond simple categorization.
  • The findings reveal novel prebiotic-bacteria interactions, expanding the understanding of gut microbiome modulation.
  • This research provides a foundation for developing targeted synbiotics based on specific prebiotic-bacterial relationships.