Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

54
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
54
CRISPR01:59

CRISPR

52.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.3K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HFpEF risk assessment using H<sub>2</sub>FPEF score in community-dwelling young Hispanic adults.

Frontiers in cardiovascular medicine·2026
Same author

Associations Between Metabolic Dysregulation and Stage B Heart Failure in Hispanic Populations.

Echocardiography (Mount Kisco, N.Y.)·2026
Same author

There is strength in numbers: the case for another model of cardiometabolic HFpEF.

American journal of physiology. Heart and circulatory physiology·2026
Same author

Nuclear receptor subfamily 4 group a member 2 induces a Warburg-like effect and promotes phospholipids synthesis in the mouse heart.

Physiological genomics·2026
Same author

A novel workflow for developing 3D mitral valve-left ventricular model using 3D-Transesophageal echocardiography.

Computers in biology and medicine·2025
Same author

Absolute concentration estimation of COVID-19 convalescent and post-vaccination IgG antibodies.

PloS one·2024
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Jul 18, 2025

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

9.7K

Liposome-Based Carriers for CRISPR Genome Editing.

Xing Yin1, Romain Harmancey1, David D McPherson1

  • 1Division of Cardiovascular Medicine, Department of Internal Medicine, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.

International Journal of Molecular Sciences
|August 26, 2023
PubMed
Summary
This summary is machine-generated.

Liposomes enhance CRISPR-Cas genome editing by improving delivery and control. This technology enables precise genetic modifications for research and therapeutic applications, advancing gene therapy.

Keywords:
CRISPR/Cas9gRNAgene deliverygene editingliposomesingle-guide RNA

More Related Videos

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.7K
Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"
09:23

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"

Published on: March 31, 2021

5.0K

Related Experiment Videos

Last Updated: Jul 18, 2025

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

9.7K
Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.7K
Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"
09:23

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"

Published on: March 31, 2021

5.0K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Therapy

Background:

  • CRISPR technology has revitalized gene therapy research.
  • Single-guide RNAs (sgRNAs) are crucial for targeted genetic modifications.
  • Efficient delivery of CRISPR/Cas components remains a significant challenge.

Purpose of the Study:

  • To explore liposomes as a delivery strategy for CRISPR/Cas and sgRNA.
  • To highlight the advantages of liposome-mediated delivery for gene editing.
  • To discuss the potential applications and future directions of liposome-based CRISPR technology.

Main Methods:

  • Review of non-viral, viral, and physical delivery methods for CRISPR/Cas9 and gRNA.
  • Focus on liposomes for enhancing CRISPR/Cas and sgRNA delivery.
  • Discussion of stimuli-responsive liposomes for controlled cargo release.

Main Results:

  • Liposomes facilitate endosomal escape and controlled release of CRISPR/Cas and gRNA.
  • Liposome-based delivery enables precise and efficient genetic modifications.
  • This approach shows promise for correcting genetic mutations and modifying immune cells.

Conclusions:

  • Liposome-based CRISPR genome editing is a valuable tool for precise genetic modification.
  • This technology has broad applications in research, biotechnology, and therapeutics.
  • Further advancements in liposome delivery will accelerate the field of gene editing.