Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

673
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
673
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.4K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mars Organic Geochemistry-Are We Alone, and Where Did We Come From?

Astrobiology·2026
Same author

A "one-two punch" strategy to reverse immunosuppressive metabolism and activate T-cell immunity for enhanced cancer checkpoint immunotherapy.

Journal of nanobiotechnology·2026
Same author

The association between steatotic liver disease and chronic kidney disease: a meta-analysis and Mendelian randomization study highlighting metabolic comorbidities.

Nutrition & diabetes·2026
Same author

Bicarbonate-Mediated Generation of H<sub>2</sub>O<sub>2</sub>, <sup>1</sup>O<sub>2</sub>, and OH<sup>*</sup> at the Hydrophobic Gas-Water Interface and Its Underlying Mechanism.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Oxidative Balance Score, Genetic Predictors of the Gut Microbiome, and the Risk of Metabolic-Associated Fatty Liver Disease: A Cohort Study.

Molecular nutrition & food research·2026
Same author

A highly efficient method for screening dual-target inhibitory peptides against angiotensin I-converting enzyme and α-amylase from Chlorella using an enzyme-immobilized affinity membrane.

International journal of biological macromolecules·2025

Related Experiment Video

Updated: Jul 18, 2025

Atomically Traceable Nanostructure Fabrication
12:35

Atomically Traceable Nanostructure Fabrication

Published on: July 17, 2015

8.8K

High-Efficiency and Reliable Value Geometric Standard: Integrated Periodic Structure Reference Materials.

Chenying Wang1,2, Di Liu2,3, Yaxin Zhang2,3

  • 1School of Instrument Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

Micromachines
|August 26, 2023
PubMed
Summary

New reference materials with integrated periodic structures offer expanded calibration capabilities for optical instruments and micro-CT. These materials enable high-efficiency, multi-value calibration with improved accuracy and diverse pattern types.

Keywords:
etching depthsintegratedline widthsperiodic structureperiodsreference materialssidewall verticality

More Related Videos

Reliable Mechanochemistry: Protocols for Reproducible Outcomes of Neat and Liquid Assisted Ball-mill Grinding Experiments
13:05

Reliable Mechanochemistry: Protocols for Reproducible Outcomes of Neat and Liquid Assisted Ball-mill Grinding Experiments

Published on: January 23, 2018

10.7K
Theoretical Calculation and Experimental Verification for Dislocation Reduction in Germanium Epitaxial Layers with Semicylindrical Voids on Silicon
06:57

Theoretical Calculation and Experimental Verification for Dislocation Reduction in Germanium Epitaxial Layers with Semicylindrical Voids on Silicon

Published on: July 17, 2020

2.2K

Related Experiment Videos

Last Updated: Jul 18, 2025

Atomically Traceable Nanostructure Fabrication
12:35

Atomically Traceable Nanostructure Fabrication

Published on: July 17, 2015

8.8K
Reliable Mechanochemistry: Protocols for Reproducible Outcomes of Neat and Liquid Assisted Ball-mill Grinding Experiments
13:05

Reliable Mechanochemistry: Protocols for Reproducible Outcomes of Neat and Liquid Assisted Ball-mill Grinding Experiments

Published on: January 23, 2018

10.7K
Theoretical Calculation and Experimental Verification for Dislocation Reduction in Germanium Epitaxial Layers with Semicylindrical Voids on Silicon
06:57

Theoretical Calculation and Experimental Verification for Dislocation Reduction in Germanium Epitaxial Layers with Semicylindrical Voids on Silicon

Published on: July 17, 2020

2.2K

Area of Science:

  • Metrology and Instrumentation
  • Materials Science
  • Nanofabrication

Background:

  • Integrated periodic structures are vital for calibrating optical instruments and micro-CT.
  • Existing reference materials have limitations in measurement range, pattern diversity, and calibration parameters.

Purpose of the Study:

  • To develop advanced integrated periodic structure reference materials with enhanced calibration features.
  • To overcome limitations of current reference materials for optical and micro-CT calibration.

Main Methods:

  • Fabrication using photolithography and inductively coupled plasma (ICP) etching.
  • MATLAB simulations to analyze structure-morphology changes during ICP etching.
  • Image processing and Fast Fourier Transform (FFT) for metrological analysis.

Main Results:

  • Achieved precise control over line widths (relative error ≤ 17.5%) and periods (relative error ≤ 1.5%).
  • Controlled etching depths from 30 to 60 μm for various grid line widths.
  • Demonstrated high side wall verticality (90° ± 0.8°, relative error < 0.9%).

Conclusions:

  • Developed versatile reference materials for high-efficiency, multi-value calibration.
  • The new materials significantly improve calibration accuracy and range for optical instruments and micro-CT.
  • Successfully addressed limitations of existing periodic structure reference materials.