Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

Subcellular Fractionation

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Biomimetic Anisotropy for Directional Transport of Liquid and Solid Samples.

Biomimetics (Basel, Switzerland)·2026

Same author

A Simple Pump-Free Approach to Generating High-Throughput Microdroplets Using Oscillating Microcone Arrays.

Micromachines·2024

Same author

A Miniaturized Archimedean Screw Pump for High-Viscosity Fluid Pumping in Microfluidics.

Micromachines·2023

Same author

A Multi-Flow Production Line for Sorting of Eggs Using Image Processing.

Sensors (Basel, Switzerland)·2023

Same author

On-Chip Organoid Formation to Study CXCR4/CXCL-12 Chemokine Microenvironment Responses for Renal Cancer Drug Testing.

Biosensors·2022

Same author

A simple acoustofluidic device for on-chip fabrication of PLGA nanoparticles.

Biomicrofluidics·2022

Search research articles

Related Experiment Video

Updated: Jul 18, 2025

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Published on: October 9, 2013

Continuous Flow Separation of Live and Dead Cells Using Gravity Sedimentation.

Adem Ozcelik¹, Sinan Gucluer¹, Tugce Keskin¹

¹Department of Mechanical Engineering, Aydin Adnan Menderes University, Aydin 09010, Türkiye.

|August 26, 2023

Summary

This summary is machine-generated.

A novel 3D-printed microfluidic device efficiently separates live and dead yeast cells, achieving over 95% purity. This cost-effective method enhances cell solutions for biomedical applications.

Keywords:

continuous flow cell separation lab-on-chip microfluidic cell separation microfluidics

More Related Videos

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Related Experiment Videos

Last Updated: Jul 18, 2025

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Published on: October 9, 2013

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Area of Science:

Biomedical Engineering
Microfluidics
Cell Biology

Background:

Cell purity is crucial for effective cell-based therapies and biomedical research.
Dead cells and debris can compromise therapeutic efficacy and experimental outcomes.
Existing cell separation methods can be expensive or inefficient.

Purpose of the Study:

To develop a cost-effective, continuous method for separating live and dead cells.
To demonstrate the efficacy of a 3D resin-printed microfluidic device for cell isolation.
To improve the purity of cell solutions for various biomedical applications.

Main Methods:

Utilized a 3D resin-printed microfluidic device for continuous cell separation.
Employed Saccharomyces cerevisiae yeast cells for experimental validation.
Conducted numerical and experimental studies to assess device performance.

Main Results:

The 3D-printed microfluidic device successfully separated live and dead yeast cells based on density differences.
Achieved separation efficiencies exceeding 95% at optimal flow rates.
Demonstrated the production of purer cell populations in the device outlets.

Conclusions:

The 3D-printed microfluidic device offers a simple, cost-effective solution for live/dead cell separation.
This technology holds significant potential for advancing cell-based therapies and single-cell studies.
3D printing in microfluidics enables customized device fabrication for diverse biomedical needs.