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Updated: Jul 18, 2025
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Severe Burn Injury in a Swine Model for Clinical Dressing Assessment
Published on: November 6, 2018
9.8K
Radiosterilized Pig Skin, Silver Nanoparticles and Skin Cells as an Integral Dressing Treatment for Burns:
Carmina Ortega-Sánchez1, Mario Pérez-Díaz1,2, Yaaziel Melgarejo-Ramírez1
1Laboratorio de Biotecnología, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Mexico City 14389, Mexico.
Pharmaceutics
|August 26, 2023
Summary
Radiosterilized pig skin (RPS) serves as an effective scaffold for culturing skin cells and stem cells, promoting enhanced wound healing in burn patients. Impregnating RPS with silver nanoparticles (AgNPs) creates an antibacterial dressing that accelerates recovery and improves extracellular matrix deposition.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing
Background:
- Radiosterilized pig skin (RPS) has been utilized as a burn dressing since the 1980s.
- Its extracellular matrix (ECM) supports mesenchymal stem cell attachment, making it suitable for cellularized constructs.
- Silver nanoparticles (AgNPs) offer an alternative to antibiotics against multidrug-resistant bacteria.
Purpose of the Study:
- To evaluate RPS as a scaffold for autologous fibroblasts (Fb), keratinocytes (Kc), and mesenchymal stem cells (MSC) in treating second-degree burns (SDB).
- To develop an antibacterial dressing by impregnating RPS with AgNPs for enhanced wound healing properties.
- To assess the efficacy and safety of RPS-AgNP constructs in preclinical and clinical settings.
Main Methods:
- Synthesized AgNPs (10 nm, rounded morphology) and characterized their antimicrobial activity against S. aureus (MIC, Kirby-Bauer assays).
- Impregnated RPS with AgNPs (RPS-AgNPs125) and evaluated wound healing in a burn mouse model, assessing ECM deposition via Masson's staining.
- Assessed cell viability (Calcein/EthD-1, MTT, SEM) and growth factor release (ELISA) of Fb, Kc, and MSC on RPS.
- Conducted a clinical trial comparing autologous RPS-Fb-Kc constructs with autologous skin grafts in an SDB patient.
Main Results:
- AgNPs exhibited antimicrobial activity against S. aureus and reduced biofilm formation.
- RPS-AgNPs125 facilitated wound healing, enhanced ECM deposition in mice, with no detectable silver in tissues.
- Fb, Kc, and MSC showed >95% viability and attachment to RPS; Kc released FGF.
- The autologous RPS-Fb-Kc construct demonstrated favorable patient recovery compared to the gold standard skin graft.
Conclusions:
- RPS is an effective scaffold for culturing Fb, Kc, and MSC, enabling the development of cellularized constructs.
- AgNP-impregnated RPS (RPS-AgNPs125) functions as a promising antibacterial wound dressing that promotes healing.
- The developed construct shows potential for improved burn treatment outcomes, offering a viable alternative to conventional grafts.
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