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An Improved Method for the Preparation of Type I Collagen From Skin
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The Efficient Extraction Method of Collagen from Deteriorated Leather Artifacts.

Li Li1, Meng Zhang1,2

  • 1Joint International Research Laboratory of Environmental and Social Archaeology, Institute of Cultural Heritage, Shandong University, Qingdao 266237, China.

Polymers
|August 26, 2023
PubMed
Summary

This study developed an efficient collagen extraction method for aged leather using a sodium hydroxide (NaOH) solution. Optimized conditions yielded high-quality collagen, crucial for artifact analysis and conservation.

Keywords:
collagenconservation of cultural relicsextraction of ancient proteinleather artifacts

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Area of Science:

  • Materials Science
  • Biochemistry
  • Analytical Chemistry

Background:

  • Collagen is vital for leather artifact analysis and conservation.
  • Traditional extraction methods struggle with degraded, denatured, or contaminated collagen in aged leather.

Purpose of the Study:

  • To develop an efficient collagen extraction strategy for aging leather.
  • To compare and optimize existing collagen extraction methods.

Main Methods:

  • Comparison of various collagen extraction techniques.
  • Orthogonal experiments to determine optimal NaOH extraction conditions (0.05 mol/L NaOH, 80°C, 12h).
  • Validation using SDS-PAGE and biological mass spectrometry.

Main Results:

  • Sodium hydroxide (NaOH) solution proved highly effective for collagen extraction from aged leather.
  • Optimal extraction conditions identified: 0.05 mol/L NaOH, 80°C, 12h.
  • SDS-PAGE showed distinct collagen bands (>20 kDa); mass spectrometry identified 124 peptides, including cattle-specific ones, even from micro-samples.

Conclusions:

  • The optimized NaOH extraction method is effective for aged leather collagen.
  • This technique enables reliable collagen identification and analysis in leather artifacts.
  • The method supports the study and conservation of historical leather materials.