Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

7.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.4K
Sperm Structure and Semen Composition01:22

Sperm Structure and Semen Composition

2.7K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
2.7K
Spermatogenesis01:41

Spermatogenesis

102.6K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.6K
Sperm Transport01:15

Sperm Transport

918
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
918

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Both L-Lactyl and D-Lactyl Enantiomers Modify Histones in Mouse Testis.

Molecular & cellular proteomics : MCP·2026
Same author

Superoxide Dismutase-Rich <i>Tetraselmis chuii</i> Supplementation Improves Semen Quality in Idiopathic Infertile Males: A Double-Blind Randomized Placebo-Controlled Trial.

The world journal of men's health·2026
Same author

Environmental and Genetic Perturbations of the Sperm Epigenome.

Advances in experimental medicine and biology·2026
Same author

Multiple Roles of Protamine Kinase SRPK1 and Phosphatase PP1γ in Sperm Development.

Proteomics·2026
Same author

Genetic variants through exome sequencing in Spanish patients affected by primary congenital glaucoma and juvenile open-angle glaucoma.

Molecular vision·2026
Same author

Familial interstitial lung disease: emerging insights into screening and genetic risk.

Frontiers in medicine·2026

Related Experiment Video

Updated: Jul 18, 2025

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
09:30

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

Published on: December 30, 2014

13.0K

Proteomics of human spermatozoa.

Judit Castillo1, Alberto de la Iglesia1, Marina Leiva1

  • 1Molecular Biology of Reproduction and Development Research Group, Departament de Biomedicina, Facultat de Medicina i Ciències de la Salut, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Fundació Clínic per a la Recerca Biomèdica, Universitat de Barcelona (UB), Barcelona, Spain.

Human Reproduction (Oxford, England)
|August 26, 2023
PubMed
Summary

Proteomics reveals thousands of sperm proteins, advancing our understanding of male fertility and infertility. Robust experimental design is crucial for reliable results and identifying potential biomarkers for reproductive health.

Keywords:
biomarkersmale infertilitymass spectrometryproteomicssemen qualitysperm

More Related Videos

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

2.5K
Analysis of Epididymal Protein Synthesis and Secretion
10:23

Analysis of Epididymal Protein Synthesis and Secretion

Published on: August 25, 2018

9.4K

Related Experiment Videos

Last Updated: Jul 18, 2025

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
09:30

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

Published on: December 30, 2014

13.0K
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

2.5K
Analysis of Epididymal Protein Synthesis and Secretion
10:23

Analysis of Epididymal Protein Synthesis and Secretion

Published on: August 25, 2018

9.4K

Area of Science:

  • Spermatozoa proteomics
  • Male reproductive biology
  • Biomarker discovery

Background:

  • Proteomic methodologies enable large-scale identification and quantification of semen proteins.
  • Over 6000 proteins in mature sperm cells have been identified, enhancing knowledge of sperm function and seminal fluid interactions.
  • Comparative proteomic studies are vital for understanding sperm maturation, functionality, and infertility.

Approach:

  • Utilizing advanced proteomic techniques for high-throughput protein identification in semen.
  • Implementing rigorous experimental design, including sample purification and quality-controlled data analysis.
  • Comparing protein profiles across fertile and infertile individuals to identify key proteins and biomarkers.

Key Points:

  • Proteomics has identified numerous proteins involved in sperm fertilizing ability.
  • Altered protein abundances are linked to infertility phenotypes like asthenozoospermia and oligozoospermia.
  • Proteomics aids in evaluating semen sample processing effects on fertility treatments.

Conclusions:

  • Sperm proteomics offers significant potential for identifying novel biomarkers for male infertility.
  • Standardized methodologies and strict data analysis are essential to overcome sample heterogeneity and variability.
  • Future research should focus on data validation and integration to maximize the clinical impact of sperm proteomic data.