Deep eutectic solvent assisted recovery of high molecular weight levan from an isolated Neobacillus pocheonensis...

The present study demonstrates the usage of deep eutectic solvent to recover microbial levan from the clarified fermented broth. The classic ethanol precipitation method for levan recovery is expensive because ethanol can be utilized as a biofuel. Production of ethanol consumes more energy and is not easily recycled. As a result, the current work concentrates on using environmentally friendly solvents for levan recovery. Deep Eutectic Solvents (DES) are greener and can replace ethanol from the microbial polysaccharides precipitation. Thus the proposed approach is environment friendly, technically feasible, reliable and economically viable. The levan was produced from a microbial isolate of aged sugarcane molasses, recovered using traditional ethanol and proposed DES (Choline Chloride and Ethylene Glycol) assisted precipitation. The levan-producing strain was characterized and identified as Neobacillus pocheonensis BPSCM4. The DES-precipitated levan has a high molecular weight of levan, 1.54 × 10⁶ KDa, compared with the ethanol-precipitated levan, 4.246 KDa. The high molecular weight of DES-precipitated levan is due to the low viscosity and hydrogen interaction of ChCl:EG with the levan present in the fermented broth. Further, the optimization enhanced the levan yield to 32.56 g/L when the sucrose concentration was 250 g/L.