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Updated: Jul 18, 2025

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events
Published on: August 27, 2019
The reversibility of cellular mechano-activation
Amy Em Beedle1, Pere Roca-Cusachs2
1Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain; Department of Physics, King's College London, London WC2R 2LS, UK.
Abstract:
The cellular microenvironment is highly heterogeneous and dynamic. Therefore, cells must be equipped with molecular tools to adapt and respond to constantly fluctuating inputs. One such input is mechanical force, which activates signalling and regulates cell behaviour in the process of mechanotransduction. Whereas the mechanisms activating mechanotransduction are well studied, the reversibility of this process, whereby cells disassemble and reverse force-activated signalling pathways upon cessation of mechanical stimulation is far less understood. In this review we will outline some of the key experimental techniques to investigate the reversibility of mechanical signalling, and key discoveries arising from them.
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