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An efficient extraction method for short single-stranded DNA from agarose gels in aptamer screening.

Chunmin Pu1, Xiaoyan Liao1, Xianming Shi2

  • 1Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China; Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.

International Journal of Biological Macromolecules
|August 26, 2023
PubMed
Summary

A new method using amino-modified silica-coated magnetic particles efficiently extracts short single-stranded DNA (ssDNA) from agarose gel. This technique shows superior performance compared to existing commercial kits for aptamer screening applications.

Keywords:
Agarose gelAmino-modified magnetic particlesAptamerDNA extractionShort single-stranded DNA

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