Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conjugated Proteins02:50

Conjugated Proteins

18.4K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.4K
Leaky Scanning02:28

Leaky Scanning

5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute respiratory distress syndrome and acute kidney injury in critically ill patients: A scoping review on this lung-kidney crosstalk.

Journal of critical care·2026
Same author

Brain aging in bipolar disorder using a neuroimaging and machine learning-derived metric: Findings from the ENIGMA BD Working Group.

Journal of affective disorders·2026
Same author

Skeletal muscle-kidney crosstalk in a cohort of critical illness survivors.

PloS one·2026
Same author

Peptidomics in Different Phases of Severe Acute Pancreatitis Model.

Proteomics. Clinical applications·2025
Same author

Stable brain PET metabolic networks using a multiple sampling scheme.

Network neuroscience (Cambridge, Mass.)·2025
Same author

Community-Acquired Acute Kidney Injury and Late Kidney Dysfunction in Survivors of COVID-19 Hospitalization.

Kidney international reports·2025

Related Experiment Video

Updated: Jul 18, 2025

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
07:53

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Published on: January 9, 2019

33.2K

Intracellular peptides in SARS-CoV-2-infected patients.

Luiz Felipe Martucci1, Rosangela A S Eichler1, Renée N O Silva1

  • 1Department of Pharmacology, Biomedical Sciences Institute, São Paulo 05508-000, Brazil.

Iscience
|August 28, 2023
PubMed
Summary

Researchers compared intracellular peptides (InPeps) in SARS-CoV-2 patients versus healthy individuals. Specific InPep levels in plasma differed, potentially predicting COVID-19 disease severity.

Keywords:
MicrobiologyPublic healthVirology

More Related Videos

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

4.7K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.1K

Related Experiment Videos

Last Updated: Jul 18, 2025

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
07:53

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Published on: January 9, 2019

33.2K
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

4.7K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.1K

Area of Science:

  • Biochemistry
  • Immunology
  • Proteomics

Background:

  • Intracellular peptides (InPeps) are generated by proteasome and peptidase activity.
  • These peptides hold significant biological and pharmacological importance.
  • Plasma InPep levels may reflect physiological changes during infection.

Purpose of the Study:

  • To compare plasma InPep concentrations between SARS-CoV-2 infected patients and non-infected individuals.
  • To identify specific InPeps that differ in concentration based on SARS-CoV-2 infection status.
  • To explore the potential of InPeps as predictive biomarkers for COVID-19 outcomes.

Main Methods:

  • Semi-quantitative analysis using isotope-labeled electrospray mass spectrometry.
  • Identification and quantification of 2,466 unique peptides in human plasma.
  • Analysis of protein-protein interaction networks and enriched pathways.

Main Results:

  • 67% of identified peptides were classified as InPeps.
  • Significant differences in specific peptide levels were observed between infected and non-infected groups.
  • A distinct COVID-19-related network was identified solely in fatally infected patients.

Conclusions:

  • Relative plasma levels of specific InPeps can distinguish between SARS-CoV-2 infected and non-infected individuals.
  • These InPep profiles may serve as predictive biomarkers for COVID-19 disease progression and severity.
  • Further research into InPep modulation could offer therapeutic or diagnostic insights.