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Updated: Jul 17, 2025

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
Published on: July 13, 2018
Preventing mitochondrial reverse electron transport as a strategy for cardioprotection
Hiran A Prag1, Michael P Murphy2,3, Thomas Krieg4
1Department of Medicine, University of Cambridge, Cambridge, CB2 0QQ, UK. hap38@cam.ac.uk.
Abstract:
In the context of myocardial infarction, the burst of superoxide generated by reverse electron transport (RET) at complex I in mitochondria is a crucial trigger for damage during ischaemia/reperfusion (I/R) injury. Here we outline the necessary conditions for superoxide production by RET at complex I and how it can occur during reperfusion. In addition, we explore various pathways that are implicated in generating the conditions for RET to occur and suggest potential therapeutic strategies to target RET, aiming to achieve cardioprotection.
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