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Updated: Jul 17, 2025
Isolation of Adipose Tissue Immune Cells
Published on: May 22, 2013
Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue
Dalila Juliana Silva Ribeiro1, Seniz Yüksel1, Andreas Dolf2
1Institute of Innate Immunity, Department of Biophysical Imaging, Medical Faculty, University of Bonn, Bonn, Germany.
Abstract:
Macrophages are one of the prominent leukocyte populations in white adipose tissue (WAT) and play an important role during WAT homeostasis and remodeling. Macrophage function in WAT is determined by ontogeny and the local tissue environment. Here, we present a protocol to analyze different macrophage populations from murine WAT using flow cytometry.
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