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Related Experiment Video

Updated: Jul 17, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
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Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging.

David Alejandro Bejarano1, Andreas Schlitzer2

  • 1Quantitative Systems Biology, LIMES Institute, University of Bonn, Bonn, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 28, 2023
PubMed
Summary

This study uses Co-Detection by Indexing (CODEX) to reveal the diverse phenotypes and locations of tissue-resident macrophages. The findings offer insights into macrophage heterogeneity and their interactions within various organs.

Keywords:
Co-Detection by Indexing (CODEX)Macrophage cellular neighborhoodsMacrophage heterogeneityMacrophage localizationMultiplexed imagingSpatial proteomics

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Area of Science:

  • Immunology
  • Cell Biology
  • Spatial Biology

Background:

  • Macrophages exhibit significant phenotypic diversity and plasticity.
  • This heterogeneity is shaped by the tissue microenvironment.

Purpose of the Study:

  • To visualize phenotypic and spatial heterogeneity of murine tissue-resident macrophages.
  • To characterize macrophage cellular microenvironments and interactions.

Main Methods:

  • Utilized Co-Detection by Indexing (CODEX), a multiplexed imaging technique.
  • Enabled simultaneous detection of multiple cellular markers for single-cell spatial identification.
  • Applied a computational pipeline for microenvironment characterization.

Main Results:

  • Visualized phenotypic and spatial heterogeneity of macrophages across multiple murine organs.
  • Characterized distinct cellular microenvironments and cell-cell interactions.
  • Identified tissue-resident hematopoietic and non-hematopoietic cells with spatial context.

Conclusions:

  • CODEX is effective for mapping macrophage heterogeneity in situ.
  • Understanding spatial interactions is crucial for macrophage function.
  • This approach provides a framework for studying tissue-specific immune cell niches.