Evaluating models and assessment techniques for understanding oral biofilm complexity
Srinivas Sulugodu Ramachandra1,2, Patricia Wright1,3, Pingping Han1,3
1Centre for Orofacial Regeneration, Rehabilitation and Reconstruction (COR3), School of Dentistry, Faculty of Health and Behavioural Sciences, The University of Queensland, Brisbane, Australia.
Microbiologyopen
|August 29, 2023
Summary
This review guides researchers on selecting oral biofilm models and assessment techniques. It covers 2D and 3D models, highlighting clinically relevant options for studying dental diseases.
Area of Science:
- Microbiology
- Dental Research
- Biomaterials Science
Background:
- Oral biofilms are complex 3D structures implicated in dental diseases.
- A wide array of biofilm models and assessment techniques exist, potentially confusing new researchers.
- Existing literature requires synthesis to guide model and technique selection.
Purpose of the Study:
- To provide a comprehensive review of oral biofilm models and assessment techniques.
- To guide novice and experienced researchers in selecting appropriate methods.
- To highlight novel 3D biofilm models, including melt electrowritten fibrous scaffolds.
Main Methods:
- Narrative review of existing scientific literature.
- Analysis of advantages and limitations of 2D and 3D biofilm models.
- Summary and analysis of various biofilm assessment methods (e.g., CFU, microscopy, molecular techniques).
Main Results:
- Established 2D models have limitations regarding growth environment and species diversity.
- Novel 3D models, especially fibrous scaffolds, offer clinically relevant biofilm growth.
- A range of assessment techniques, from culture-based to next-generation sequencing, are evaluated.
Conclusions:
- Selecting appropriate oral biofilm models and assessment techniques is crucial for relevant research.
- Advanced 3D models and methodologies can enhance the study of clinically relevant biofilms.
- This review serves as a guide for optimizing oral biofilm research strategies.
