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Published on: April 23, 2012
Fungal Sterol Analyses by Gas Chromatography-Mass Spectrometry Using Different Derivatives
Aurélie Mossion1, Isabelle Ourliac-Garnier2, Gaetane Wielgosz-Collin3
1Nantes Université, Institut des Substances et Organismes de la Mer, ISOMer, Nantes, France. aurelie.mossion@univ-nantes.fr.
Abstract:
Sterols are the main components of the fungal membrane. Their study can be used to describe the chemical biodiversity among the strains and species or to work on antifungal treatment. Those molecules can be analyzed by gas chromatography coupled with mass spectrometry (GC-MS) as free molecules or after derivation as acetate or trimethylsilyl ether (TMSi). Here we describe how to extract sterols from fungal biomass according to its physiological form and the culture conditions (liquid and solid media). The different methodologies that can be used to obtain free sterols, acetate, and/or TMSi derivatives are presented. Identification keys using the fragmentation at 70 eV are also described.
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