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Related Experiment Video

Updated: Jul 17, 2025

Fast and Accurate Exhaled Breath Ammonia Measurement

Fast and Accurate Exhaled Breath Ammonia Measurement

Published on: June 11, 2014

MEASURE: Multiplex Exhaled Breath Condensate - Scanning Using Rapid Electro-Analytics.

Ashlesha Bhide¹, Mohammed A Eldeeb¹, Madhavi Pali¹

¹Department of Bioengineering, The University of Texas at Dallas, Richardson, Texas 75080, United States.

|August 29, 2023

Summary

A new electrochemical sensor, READ 2.0, noninvasively detects inflammatory biomarkers in exhaled breath condensate (EBC) for respiratory disorder monitoring. This platform offers a promising alternative to invasive methods for predicting disease severity and survival rates.

Keywords:

IL-1β IL-6 IL-8 electrochemical impedance spectroscopy exhaled breath condensate hs-CRP inflammation point-of-care

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Area of Science:

Biomedical Engineering
Respiratory Medicine
Analytical Chemistry

Background:

Exhaled breath condensate (EBC) is a noninvasive source for detecting respiratory disorder biomarkers.
Current diagnostic methods for airway inflammation are invasive and challenging for monitoring.
Cytokine biomarkers like IL-6, IL-1β, IL-8, and hs-CRP are crucial indicators in respiratory diseases.

Purpose of the Study:

To develop and validate a novel 16-plexed electrochemical platform, READ 2.0, for multiplexed biomarker detection in EBC.
To assess the performance characteristics of the READ 2.0 sensor for key inflammatory biomarkers.
To evaluate the potential of EBC-based biomarker monitoring for predicting respiratory disorder severity and outcomes.

Main Methods:

Utilized a 16-plexed electrochemical sensor (READ 2.0) for simultaneous detection of IL-6, IL-1β, IL-8, and hs-CRP in EBC.
Determined sensor dynamic ranges, limits of detection, and accuracy.
Performed analytical validation against ELISA, assessing correlation and bias, and evaluated sensor precision and stability.

Main Results:

The READ 2.0 platform demonstrated sensitive detection with dynamic ranges up to 243 pg/mL and low limits of detection (1 pg/mL for IL-6/IL-1β, 3 pg/mL for IL-8/hs-CRP).
Detection accuracies ranged from approximately 85% to 100%, with high correlation (R² > 0.96) and low bias compared to ELISA.
The sensor exhibited excellent precision (%CV < 7%) and stability (variation < 10% over 6 hours).

Conclusions:

The READ 2.0 electrochemical platform enables reliable, noninvasive multiplexed detection of key inflammatory biomarkers in EBC.
This technology shows significant promise for predicting respiratory disorder severity and survival rates.
EBC biomarker monitoring using READ 2.0 can serve as a vital reference point for managing respiratory conditions.