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Loss of NDUFS1 promotes gastric cancer progression by activating the mitochondrial ROS-HIF1α-FBLN5 signaling pathway

Tao Chen1, Dongbao Li1, Yunliang Wang1

  • 1Department of General Surgery, the First Affiliated Hospital of Soochow University, 215006, Suzhou, China.

British Journal of Cancer
|August 29, 2023
PubMed
Abstract

Insights

NDUFS1 downregulation promotes gastric cancer (GC) progression. This occurs through activation of the mitochondrial reactive oxygen species (mROS)-hypoxia-inducible factor 1α (HIF1α)-fibulin 5 (FBLN5) pathway, driving cancer cell growth and metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • NDUFS1's role in human cancers is recognized, but its specific impact on gastric cancer (GC) remains unclear.
  • Gastric cancer (GC) is a significant global health concern with complex underlying molecular mechanisms.

Purpose of the Study:

  • To investigate the role and mechanism of NDUFS1 in gastric cancer (GC) progression.
  • To elucidate the signaling pathway involved in NDUFS1-mediated GC development.

Main Methods:

  • Western blot, immunohistochemistry, and bioinformatics analysis to assess NDUFS1 expression in GC.
  • In vitro (CCK-8, colony formation, Transwell assays) and in vivo (mouse xenograft) experiments to evaluate NDUFS1's functional impact.
  • Confocal microscopy to observe NDUFS1 localization and mitochondrial reactive oxygen species (mROS) levels.

Main Results:

  • NDUFS1 expression was significantly downregulated in GC tissues and cell lines.
  • NDUFS1 overexpression suppressed GC cell proliferation, migration, invasion, and tumor growth in vivo.
  • NDUFS1 reduction activated the mROS-hypoxia-inducible factor 1α (HIF1α) pathway, leading to increased fibulin 5 (FBLN5) expression.

Conclusions:

  • NDUFS1 downregulation is a key driver of gastric cancer (GC) progression.
  • The mROS-HIF1α-FBLN5 signaling pathway is critically involved in NDUFS1-mediated GC development.
  • Targeting NDUFS1 or this pathway may offer therapeutic strategies for gastric cancer.

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