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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jul 17, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Highly multiplexed mRNA quantitation with CRISPR-Cas13.

Brian Kang¹, Jiayu Zhang¹, Michael P Schwoerer¹

¹Department of Molecular Biology, Princeton University, Princeton, NJ 08544.

Biorxiv : the Preprint Server for Biology

|August 30, 2023

Summary

This summary is machine-generated.

Quantitative Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids (qCARMEN) enables high-throughput, cost-effective RNA quantitation. This method profiles over 4,500 gene-sample pairs, advancing multi-gene studies.

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Last Updated: Jul 17, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Published on: August 3, 2011

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Existing RNA quantitation methods present a trade-off between high-throughput and cost-effectiveness.
Profiling the transcriptome is often expensive, while low-cost methods are limited in scope.

Approach:

Introduced quantitative Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids (qCARMEN), a novel RNA quantitation technique.
Leveraged CRISPR-Cas13's programmable RNA-targeting for multiplexed nucleic acid evaluation.
Enabled quantification of over 4,500 gene-sample pairs in a single experiment.

Key Points:

Demonstrated qCARMEN's utility in studying interferon-stimulated gene (ISG) responses during interferon stimulation and flavivirus infection.
Observed isoform switching kinetics during epithelial-mesenchymal transition.
Achieved performance comparable to gold-standard methods.

Conclusions:

qCARMEN offers a simple, inexpensive, and scalable solution for RNA quantitation.
Enhances the feasibility of multi-gene kinetic studies, gene regulatory network analysis, and combinatorial genetic screens.
Expands applications in transcriptomics with high throughput and cost-efficiency.