Related Experiment Video
Updated: Jul 17, 2025
11:44
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
7.7K
Endothelial TMEM16F lipid scramblase regulates angiogenesis
Ke Zoe Shan1, Trieu Le1,2, Pengfei Liang1
1Department of Biochemistry, Duke University, School of Medicine, Durham, NC 27710, USA.
Biorxiv : the Preprint Server for Biology
|August 30, 2023
Summary
Endothelial TMEM16F regulates angiogenesis by impacting VE-cadherin and Src kinase signaling. TMEM16F deficiency impairs blood vessel development in mice and human cells.
Area of Science:
- Cell Biology
- Molecular Biology
- Physiology
Background:
- TMEM16 Ca2+-activated lipid scramblases (CaLSase) are crucial for membrane dynamics.
- TMEM16F's role in endothelial biology and angiogenesis is not well understood.
Approach:
- Investigated TMEM16F function in endothelial cells using in vitro and in vivo models.
- Utilized TMEM16F deficient mice and siRNA knockdown in human umbilical vein endothelial cells.
- Analyzed the impact on angiogenesis, VE-cadherin expression, and Src kinase phosphorylation.
Key Points:
- Endothelial TMEM16F is essential for regulating angiogenesis.
- TMEM16F deficiency impairs retinal angiogenesis in mice.
- TMEM16F knockdown compromises in vitro angiogenesis and affects VE-cadherin and Src kinase signaling.
Conclusions:
- TMEM16F plays a novel role in regulating angiogenesis through intracellular signaling pathways.
- TMEM16F influences angiogenesis by modulating VE-cadherin phosphorylation and expression via Src kinase.
Related Concept Videos
01:24Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
01:10Mechanism of Angiogenesis
5.6K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.6K
01:19Membrane Asymmetry Regulating Transporters
4.6K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.6K
01:17Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K