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Tagging and Fusion Proteins

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins

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Expression, Purification and Characterization of Functional Teduglutide Using GST Fusion System in Prokaryotic Cells.

Ali Akbar Alizadeh¹, Saba Rasouli^2,3, Omid Jamshidi Kandjani¹

¹Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Advanced Pharmaceutical Bulletin

|August 30, 2023

Summary

This study presents a recombinant DNA method for producing teduglutide, a treatment for short bowel syndrome (SBS). The method successfully expressed, purified, and confirmed the functionality and structure of teduglutide for potential large-scale manufacturing.

Keywords:

Affinity chromatography Peptide Recombinant technology SBS Size exclusion chromatography Teduglutide

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Area of Science:

Biotechnology
Molecular Biology
Drug Development

Background:

Short bowel syndrome (SBS) is a malabsorptive condition.
Teduglutide is an FDA-approved therapeutic for SBS.
Efficient production methods are crucial for drug accessibility.

Purpose of the Study:

To develop a recombinant DNA technology approach for teduglutide production.
To establish a scalable method for manufacturing teduglutide.

Main Methods:

Cloning the teduglutide gene into a pGEX-2T vector with a Factor Xa cleavage site.
Overexpressing the GST-teduglutide fusion protein in E. coli BL21 (DE3).
Purification using glutathione sepharose affinity chromatography, on-column Factor Xa digestion, and size exclusion chromatography.

Main Results:

Successfully expressed and purified teduglutide with high structural integrity confirmed by circular dichroism and mass spectroscopy.
Demonstrated teduglutide's biological activity through in vitro assays, showing significant induction of Caco2 cell proliferation.
Achieved a primarily alpha-helical structure (>50%) for the produced teduglutide.

Conclusions:

The developed recombinant DNA technology approach enables successful expression, purification, and functional validation of teduglutide.
The experimental-scale method is suitable for adaptation to pilot-scale and industrial-scale teduglutide production.
This study provides a foundation for efficient and scalable manufacturing of teduglutide for SBS treatment.