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Updated: Jul 17, 2025
Murine Model of Intestinal Ischemia-reperfusion Injury
Published on: May 11, 2016
miR-379-5P INHIBITION ENHANCES INTESTINAL EPITHELIAL PROLIFERATION AND BARRIER FUNCTION RECOVERY AFTER
Zirui Jia, Yuhang Wang, Jiacheng Gao
1Department of Gastrointestinal Surgery, The Dalian Municipal Central Hospital Affiliated to Dalian Medical University, Dalian, China.
Abstract:
Gut barrier dysfunction caused by intestinal ischemia/reperfusion (I/R) injury is associated with substantial death and morbidity. In this research, the role of microRNAs (miRNAs) in regulating intestinal I/R injury was investigated. We used miRNA sequencing to analyze clinical ischemic and normal intestinal samples. Through bioinformatics analysis based on sequencing results, we found that upregulated miRNAs inhibited epithelial barrier function and cell proliferation, with miR-379-5p being the most significantly upregulated in the ischemic intestine. Further studies confirmed the role of miR-379-5p through experiments in the human ischemic intestine, the mouse I/R injury model in vivo , and cell hypoxia/reoxygenation models in vitro . Inhibiting miR-379-5p increased epithelial cell proliferation and improved barrier function after I/R injury. We also identified eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) as a downstream target gene of miR-379-5p through bioinformatics prediction and experimental verification. The findings suggest that inhibiting miR-379-5p could improve intestinal epithelial cell proliferation and barrier function by targeting EIF4G2. The goal of this study was to find a potential target for treating I/R injury in the intestine, as well as to prevent and mitigate the damage caused.
Insights
Inhibiting miR-379-5p improves intestinal barrier function and cell proliferation following ischemia/reperfusion (I/R) injury. This microRNA targets EIF4G2, offering a potential therapeutic strategy for I/R damage.
Area of Science:
- Gastroenterology
- Molecular Biology
- Biochemistry
Background:
- Intestinal ischemia/reperfusion (I/R) injury leads to gut barrier dysfunction, causing significant mortality and morbidity.
- MicroRNAs (miRNAs) are implicated in regulating cellular processes relevant to I/R injury.
Purpose of the Study:
- To investigate the role of miRNAs in intestinal I/R injury.
- To identify specific miRNAs and their targets involved in I/R-induced gut barrier dysfunction.
- To explore potential therapeutic targets for mitigating I/R injury.
Main Methods:
- miRNA sequencing of clinical ischemic and normal intestinal samples.
- Bioinformatics analysis to identify dysregulated miRNAs and predict targets.
- In vivo (mouse I/R model) and in vitro (cell hypoxia/reoxygenation) experiments to validate findings.
- Experimental verification of miRNA-target interactions.
Main Results:
- miR-379-5p was significantly upregulated in ischemic intestines and inhibited epithelial barrier function and cell proliferation.
- Inhibition of miR-379-5p enhanced epithelial cell proliferation and barrier function in I/R injury models.
- Eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) was identified as a direct downstream target of miR-379-5p.
Conclusions:
- miR-379-5p plays a detrimental role in intestinal I/R injury by suppressing epithelial barrier function and proliferation.
- Targeting miR-379-5p, potentially by modulating its interaction with EIF4G2, represents a promising therapeutic strategy for intestinal I/R injury.
