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Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case
Milene Dias Miranda1, Gabriela Cardoso Caldas1,2, Vivian Neuza Ferreira1
1Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Morfologia e Morfogênese Viral, Rio de Janeiro, RJ, Brasil.
Memorias Do Instituto Oswaldo Cruz
|August 30, 2023
Summary
Researchers isolated Monkeypox (Mpox) virus from a Brazilian patient, observing its effects on cell structures. This study aids in developing new antiviral drugs and diagnostic strategies for Mpox infections.
Area of Science:
- Virology
- Cell Biology
- Infectious Diseases
Background:
- The 2023 Monkeypox (Mpox) outbreak has affected over 100 countries, with Brazil reporting a high number of cases.
- Understanding Mpox virus is crucial for global health, especially in regions with significant case numbers.
Purpose of the Study:
- To investigate the effects of Mpox virus infection in a cellular model.
- To aid in the diagnosis of Mpox and develop strategies for viral inhibition.
Main Methods:
- Virus isolation from a human clinical sample from Brazil.
- Viral growth assessment using plaque-forming units (PFU) assay.
- Analysis of viral infection using reverse transcriptase polymerase chain reaction (RT-PCR) and transmission electron microscopy (TEM).
Main Results:
- Successful isolation of Mpox virus in Vero cell culture from a Brazilian patient.
- Demonstration of Mpox virus-induced effects on cellular structures via TEM.
- Characterization of viral replication kinetics in a cell model.
Conclusions:
- Understanding Mpox virus's impact on cellular structures and replication is key.
- Findings may inform the development of novel antiviral drugs and therapeutic strategies.
- This research contributes to better diagnostic and treatment approaches for Mpox.
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