Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

4.9K
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
4.9K
ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

3.8K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
3.8K
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

8.2K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
8.2K
Open and closed-loop control systems01:17

Open and closed-loop control systems

801
Control systems are foundational elements in automation and engineering. They are broadly categorized into open-loop and closed-loop systems. These classifications hinge on the presence or absence of feedback mechanisms, significantly influencing the system's performance, complexity, and application.
An open-loop control system operates without feedback from the output. It consists of two primary elements: the controller and the controlled process. The controller receives an input signal...
801
PID Controller01:19

PID Controller

142
Proportional-Integral-Derivative (PID) controllers are widely used in various control systems to enhance stability and performance. In a thermostat, it adjusts heating or cooling based on the temperature difference between the actual and desired levels. They are often used in automotive speed systems, effectively managing sudden speed changes while maintaining a constant speed under varying conditions. On the other hand, PI controllers, commonly employed in voltage regulation, enhance stability...
142

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A guide to soft colloidal lithography: Advances in microgels at fluid interfaces, preparation methods and applications of 2D microgel monolayers.

Advances in colloid and interface science·2025
Same author

Use of emulsifying plant protein hydrolysates from winery, whiskey and brewery by-products for the development of echium oil delivery emulsions.

International journal of biological macromolecules·2025
Same author

In-situ characterization of microgel monolayers: Controlling isostructural phase transitions for homogeneous crystal drying patterns.

Journal of colloid and interface science·2025
Same author

An in-depth interfacial study uncovering the effect of emulsifier mixes on small intestinal in vitro lipid digestion kinetics.

Food chemistry·2025
Same author

Interfacial behaviour of human bile and its substitution for in vitro lipolysis studies.

Food research international (Ottawa, Ont.)·2024
Same author

The bile salt/phospholipid ratio determines the extent of in vitro intestinal lipolysis of triglycerides: Interfacial and emulsion studies.

Food research international (Ottawa, Ont.)·2024

Related Experiment Video

Updated: Jul 17, 2025

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
09:08

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications

Published on: August 30, 2018

12.5K

Autotitrator based on an Arduino Open Source Pump.

Teresa Del Castillo-Santaella1,2, Julia Maldonado-Valderrama1,3, Miguel Angel Fernandez-Rodriguez1,3

  • 1Department of Applied Physics, Faculty of Sciences, University of Granada, Campus de Fuentenueva s/n, 18071 Granada, Spain.

Hardwarex
|August 31, 2023
PubMed
Summary
This summary is machine-generated.

We developed a low-cost, open-source automated titrator using an Arduino-based pump. This affordable device accurately measures acidic or basic groups in solutions, demonstrated by analyzing olive oil lipolysis.

Keywords:
ArduinoAutomated titrationpH meter

More Related Videos

Optimization, Test and Diagnostics of Miniaturized Hall Thrusters
12:22

Optimization, Test and Diagnostics of Miniaturized Hall Thrusters

Published on: February 16, 2019

9.0K
Modeling and Experimental Analysis of the Single-Shaft Coaxial Motor-Pump Assembly in Electrohydrostatic Actuators
08:59

Modeling and Experimental Analysis of the Single-Shaft Coaxial Motor-Pump Assembly in Electrohydrostatic Actuators

Published on: June 13, 2022

2.6K

Related Experiment Videos

Last Updated: Jul 17, 2025

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
09:08

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications

Published on: August 30, 2018

12.5K
Optimization, Test and Diagnostics of Miniaturized Hall Thrusters
12:22

Optimization, Test and Diagnostics of Miniaturized Hall Thrusters

Published on: February 16, 2019

9.0K
Modeling and Experimental Analysis of the Single-Shaft Coaxial Motor-Pump Assembly in Electrohydrostatic Actuators
08:59

Modeling and Experimental Analysis of the Single-Shaft Coaxial Motor-Pump Assembly in Electrohydrostatic Actuators

Published on: June 13, 2022

2.6K

Area of Science:

  • Analytical Chemistry
  • Open Source Hardware

Background:

  • Acid-base titration is crucial for quantifying acidic/basic groups in solutions.
  • Automated titration systems offer improved precision and efficiency.
  • Existing commercial automated titrators can be prohibitively expensive.

Purpose of the Study:

  • To develop and validate a low-cost, open-source automated titrator.
  • To demonstrate the device's utility in quantitative analysis.
  • To provide a customizable and affordable alternative to commercial systems.

Main Methods:

  • Modification of an Arduino-based Open Source Pump (OSPump) to function as an automated titrator.
  • Integration with a Metrohm 713 pH meter for pH monitoring.
  • Validation using two case studies, including olive oil lipolysis analysis.

Main Results:

  • The OSPump Titrator was successfully developed as a functional automated titration system.
  • The device achieved accurate quantitative analysis, validated by lipolysis studies.
  • The cost of the OSPump Titrator is approximately 50 times lower than comparable second-hand commercial units.

Conclusions:

  • The OSPump Titrator offers a cost-effective and customizable solution for automated acid-base titration.
  • This open-source approach democratizes access to automated analytical instrumentation.
  • The device is suitable for various quantitative analyses, including complex emulsion studies.