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Heterogenous pathogen profile associated with acute conjunctivitis in Nepal.
Meenu Chaudhary1, Sanjeeta Sitaula1, Kevin Ruder2
1B.P. Koira la Lions Center for Ophthalmic Studies, Institute of Medicine, Tribhuvan University, Kathmandu 44600, Nepal.
International Health
|August 31, 2023
Summary
Infectious conjunctivitis in Nepal is often caused by RNA viruses, including SARS-CoV-2. Metagenomic RNA sequencing revealed these viruses as the predominant pathogens, contrary to expectations.
Area of Science:
- Ophthalmology
- Virology
- Microbiology
Background:
- Infectious conjunctivitis is a prevalent condition in Nepal.
- Understanding the etiological agents is crucial for effective management.
Purpose of the Study:
- To investigate the causative pathogens of acute infectious conjunctivitis in Nepal.
- To identify the predominant pathogen classes using advanced sequencing techniques.
Main Methods:
- Prospective recruitment of 60 patients with acute infectious conjunctivitis in Kathmandu, Nepal.
- Collection of conjunctival and nasal swabs.
- Metagenomic RNA deep sequencing (RNA-seq) for pathogen identification.
Main Results:
- Pathogens were identified in 55% of the studied cases.
- RNA viruses were the most frequently detected pathogen class.
- Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was the most common RNA virus identified.
Conclusions:
- The spectrum of infectious conjunctivitis pathogens can vary geographically.
- RNA viruses, unexpectedly, predominated in this cohort.
- Metagenomic RNA sequencing is a powerful tool for identifying novel and unexpected pathogens, including RNA viruses.
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