[Comparative studies of hemoglobin methods for the determination of blood hemoglobin concentration]

The interference and clarifying effect of the non-ion detergents "Vero" "Verolux", "Alva" and "Alkaryl" on the formation of cyanhemiglobin and azihemiglobin complexes in slightly alkaline media (pH 7.2) (methods of von Kampen and Zijlstra and of Vancetti and Nardeschi) are studied by the investigation of standard curves, spectral characteristics of colour complex and time effect. The high degree of correlation (r = +0.960 to +0.999) with the parallel investigations with Nonidet P 40 and the negligible interference of the detergents studied provide grounds to substitute Nonidet P 40 in the recommended prescriptions for the preparation of transforming solutions for "Vero", "Verolux", "Alva" and "Aklaryl", especially if some of them (for instance Vero) is prepared with a purity degree, meeting the analytical needs of laboratory practice.