Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mitochondrial Complex I Modulator Restores Network Resilience in Advanced Alzheimer's Disease Through Metabolic Reprogramming.

bioRxiv : the preprint server for biology·2026

Same author

Associations of testosterone, sex hormone-binding globulin, and related hormones with risks of cancer death, incident cancer, and incident prostate cancer in men: individual participant data meta-analyses.

The lancet. Healthy longevity·2026

Same author

Corona: A Virtual Mass Spectrometer for the Development of Real-Time Mass Spectrometry Software.

Analytical chemistry·2026

Same author

Author Correction: Community benchmarking and evaluation of human unannotated microprotein detection by mass spectrometry based proteomics.

Nature communications·2026

Same author

Blueprint 2: A self-directed mobile adaptive coping skills intervention to improve psychological distress symptoms among cardiorespiratory failure survivors study protocol.

Contemporary clinical trials·2026

Same author

Collateral lethality of NAD kinase in 1p36 deleted tumors drives vulnerability to NAD kinase 2 depletion.

BBA advances·2026

Same journal

The influence of aging on graded peripheral venous return and complex blood flow features through human veins.

GeroScience·2026

Same journal

Age-related differences in motor unit discharge rate modulation across contraction levels.

GeroScience·2026

Same journal

Study protocol for FAXAge: a randomized, controlled clinical trial of fasting and exercise to slow aging in humans.

GeroScience·2026

Same journal

Long-term intake of statins or renin-angiotensin system inhibitors: associations with sarcopenia-related parameters in older people.

GeroScience·2026

Same journal

The shaky voice of aging localized to the larynx: dissociation of frequency and amplitude tremor.

GeroScience·2026

Same journal

Plasma proteins associated with disability and mortality risks in Japanese community-dwelling octogenarians.

GeroScience·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 17, 2025

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

Proteomic changes induced by longevity-promoting interventions in mice.

Adam R Burns¹, Jack Wiedrick², Alicia Feryn²

¹Biostatistics & Design Program, Oregon Health & Science University, Portland, OR, USA. burnsad@ohsu.edu.

|August 31, 2023

Summary

This summary is machine-generated.

Researchers analyzed proteome changes in mice undergoing lifespan-extending interventions. Key findings point to proteins involved in peroxisomal oxidation and fatty acid metabolism as potential targets for promoting healthy aging.

Keywords:

Longevity Mass spectrometry Mouse model Proteomics

More Related Videos

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Published on: December 9, 2022

Related Experiment Videos

Last Updated: Jul 17, 2025

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Published on: December 9, 2022

Area of Science:

Biochemistry
Gerontology
Proteomics

Background:

Aging is a complex process influenced by various genetic and environmental factors.
Interventions like caloric restriction and specific drug treatments are known to extend lifespan in model organisms.
Understanding the molecular mechanisms underlying lifespan extension is crucial for developing strategies to promote healthy aging.

Purpose of the Study:

To investigate proteome alterations in response to multiple lifespan-extending interventions in mice.
To identify shared protein changes across different interventions that may indicate common longevity pathways.
To uncover novel protein candidates and biological mechanisms associated with enhanced longevity.

Main Methods:

Utilized mouse models subjected to seven distinct lifespan-extending interventions.
Employed high-throughput proteomics to analyze protein concentration changes in liver, kidney, and muscle tissues.
Compared proteomic profiles across interventions to identify common and distinct molecular responses.

Main Results:

Each intervention induced variable proteome changes, with the liver showing the strongest responses.
Limited concordance in protein responses was observed across different tissue types.
No single protein was affected by all interventions, but a set of proteins involved in peroxisomal oxidation and fatty acid metabolism responded to multiple interventions.

Conclusions:

Lifespan-extending interventions trigger diverse proteomic alterations, suggesting multiple pathways contribute to longevity.
Proteins involved in peroxisomal oxidation and fatty acid metabolism represent promising targets for interventions aimed at promoting healthy aging.
These findings provide a foundation for future research into therapeutic strategies for age-related diseases and lifespan extension.