Related Experiment Video

Updated: Aug 7, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

[Amplification of myc-specific sequences in human colonic cancer]

O M Serova, T V Shpatar', S N Fedorov

    Voprosy Onkologii
    |January 1, 1986
    PubMed

    Insights

    Southern blot analysis revealed amplified v-myc oncogene DNA sequences in one of five large bowel tumors. This suggests potential genetic alterations in multiple neoplasia, with differing restriction fragment sizes observed.

    Area of Science:

    • Molecular biology
    • Oncology
    • Genetics

    Context:

    • Investigating genetic alterations in large bowel multiple neoplasia.
    • Examining DNA from tumor and adjacent mucosal tissues.

    Purpose:

    • To identify oncogene amplifications in large bowel tumors.
    • To analyze variations in myc gene family DNA sequences.

    Summary:

    • Southern blot analysis was performed on DNA from a patient with large bowel multiple neoplasia.
    • Amplification of v-myc oncogene-related DNA sequences was detected in 1 out of 5 tumors.
    • Restriction fragment analysis showed size differences between amplified myc sequences and endogenous c-myc/N-myc loci.

    Impact:

    • Highlights potential role of v-myc amplification in large bowel neoplasia.
    • Suggests genomic instability or specific oncogenic pathways in this condition.
    • Provides data for further research into myc family gene involvement in colorectal cancers.

    More Related Videos

    Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing15:17

    Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

    Published on: September 25, 2011

    Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue06:59

    Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

    Published on: October 13, 2013

    Related Concept Videos

    Abnormal Proliferation02:23

    Abnormal Proliferation

    Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...