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[Monoclonal immunoglobulins to the antigens of the Japanese encephalitis virus]
Six hybridomas of the EJ series producing monoclonal antibodies to Japanese encephalitis virus antigens were generated by hybridization of immune splenocytes with the parental line of mouse myeloma cells NS-0, and one hybridoma (EJ-10) with the X63-Ag8/653 line. Among 7 species of monoclonal antibodies examined by Ouchterlony method, 3 were identified as IgM and 4 as IgG. The highest clone-producing efficacy was shown by hybridoma EJ-10 generated on the basis of X-653 cells and the least by hybridoma EJ-20. The hybrid cells readily established in the cavity of mice producing ascitic tumors in 37%-86% cases. Among the derived clones, two were found, EJ-4 (IgG) and EJ-19 (IgM), to possess a high growth potential, satisfactory clone-producing efficacy, a high per cent of positive clones in recloning, and stable production of antiviral monoclonal antibody. Hybridomas EJ-4 and EJ-19 demonstrated a marked capacity for mouse-to-mouse transmission in serial passages providing for preparative accumulation of these monospecific immunoglobulins.
Six hybridomas of the EJ series producing monoclonal antibodies to Japanese encephalitis virus antigens were generated by hybridization of immune splenocytes with the parental line of mouse myeloma cells NS-0, and one hybridoma (EJ-10) with the X63-Ag8/653 line. Among 7 species of monoclonal antibodies examined by Ouchterlony method, 3 were identified as IgM and 4 as IgG. The highest clone-producing efficacy was shown by hybridoma EJ-10 generated on the basis of X-653 cells and the least by hybridoma EJ-20. The hybrid cells readily established in the cavity of mice producing ascitic tumors in 37%-86% cases. Among the derived clones, two were found, EJ-4 (IgG) and EJ-19 (IgM), to possess a high growth potential, satisfactory clone-producing efficacy, a high per cent of positive clones in recloning, and stable production of antiviral monoclonal antibody. Hybridomas EJ-4 and EJ-19 demonstrated a marked capacity for mouse-to-mouse transmission in serial passages providing for preparative accumulation of these monospecific immunoglobulins.