Related Experiment Videos
[Use of a microfiltration method in producing inactivated influenza vaccine]
Abstract:
The process of microfiltration carried out in the regimen of destruction of concentrational polarization layer decreases considerably the retention of admixture proteins in filtrational concentration of virus suspension. This allows the membrane technology to be used not only for concentration but also for profound purification of the virus-containing allantoic fluid for preparation of inactivated influenza virus. Using the microfiltration method, vaccine preparations of any influenza virus strain may be obtained without loss of biological activity. The resultant vaccine is highly immunogenic and safe for man. The filtration technology makes it possible to restore the preparations discarded because of low virus titres or excess protein content.
Insights
This study shows how microfiltration can purify and concentrate virus suspensions, leading to safe and effective inactivated influenza virus vaccines. This method improves vaccine yield and quality, even for previously discarded preparations.
Area of Science:
- Virology
- Biotechnology
- Membrane Science
Background:
- Influenza virus vaccine production often faces challenges with protein contamination and low yields.
- Current methods may result in loss of viral biological activity or require discarding batches with suboptimal virus titers or high protein content.
Purpose of the Study:
- To investigate the use of microfiltration with a destroyed concentrational polarization layer for purifying virus suspensions.
- To evaluate the efficacy of this method in producing inactivated influenza virus vaccines with high immunogenicity and safety.
Main Methods:
- Microfiltration was employed under conditions designed to disrupt the concentrational polarization layer.
- Virus suspensions were concentrated and purified using this optimized microfiltration technique.
- The biological activity, immunogenicity, and safety of the resulting inactivated influenza virus vaccine were assessed.
Main Results:
- The microfiltration process significantly reduced the retention of admixture proteins during virus suspension concentration.
- This enabled both concentration and profound purification of virus-containing allantoic fluid.
- Vaccine preparations of all influenza virus strains were obtained without loss of biological activity, demonstrating high immunogenicity and safety.
Conclusions:
- Optimized microfiltration is an effective technology for producing high-quality inactivated influenza virus vaccines.
- This method enhances purification, preserves viral biological activity, and improves vaccine yield.
- The technology allows for the recovery of valuable vaccine preparations previously deemed unusable due to low virus titers or excess protein.