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[Synthesis of measles virus proteins in chronically infected human cell cultures]
Abstract:
Synthesis of measles virus proteins in primary and chronic infection of L-41 and HEP-2 cells was studied by radioimmunoprecipitation test and polyacrylamide gel electrophoresis. Synthesis of measles virus main structural proteins, H, P, NP, and M, was found to occur in chronically infected cells. Persistence of measles virus in the systems under study was shown not to be accompanied by changes in electrophoretic mobility of virus polypeptides as compared with proteins synthesized in primary infection. NP protein of the original virus, however, differed significantly from NP protein of the persisting virus in its charge which indicated aminoacid replacements apparently due to mutations of the appropriate region of the virus genome. The mechanism of measles virus persistence in these systems was shown not to be associated with disorders in matrix protein synthesis. Use of monoclonal antibodies in fluorescent antibody technique and radioimmunoprecipitation revealed the stability of a number of antigenic determinants in persisting measles virus in L-41 and HEP-2 cell cultures.
Insights
Measles virus proteins are synthesized during chronic infection, with NP protein mutations observed. Viral persistence doesn't alter electrophoretic mobility or matrix protein synthesis.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Context:
- Measles virus can establish persistent infections in cell cultures.
- Understanding the molecular mechanisms of viral persistence is crucial for developing antiviral strategies.
Purpose:
- To investigate the synthesis and characteristics of measles virus proteins during primary and chronic infections.
- To determine if viral persistence is associated with changes in protein structure or synthesis.
Summary:
- Measles virus structural proteins (H, P, NP, M) are synthesized in chronically infected L-41 and HEP-2 cells.
- While electrophoretic mobility of viral polypeptides remains unchanged, NP protein shows charge differences, indicating mutations.
- Viral persistence is not linked to matrix protein synthesis defects, and antigenic determinants are stable.
Impact:
- This study elucidates molecular aspects of measles virus persistence.
- Findings suggest specific mutations in NP protein contribute to measles virus chronicity.
- Identifies stable antigenic sites on persistent measles virus, potentially relevant for vaccine development or diagnostics.