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Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

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Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Published on: June 16, 2019

Generating hematopoietic cells from human pluripotent stem cells: approaches, progress and challenges.

Haiqiong Zheng^1,2,3,4, Yijin Chen^1,2,3,4, Qian Luo^1,2,3,4

¹Bone Marrow Transplantation Center, the First Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, 310012, China.

Cell Regeneration (London, England)

|September 1, 2023

Summary

Human pluripotent stem cells (hPSCs) can generate many blood cell types. Generating large-scale, mature, functional cells for clinical use remains challenging, but recent methods offer solutions.

Keywords:

Blood cells Hematopoietic differentiation Hematopoietic stem cells Human pluripotent stem cells

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Area of Science:

Stem cell biology
Hematology
Regenerative medicine

Background:

Human pluripotent stem cells (hPSCs) are a promising source for clinical blood cell production.
Significant progress has been made in differentiating hPSCs into various blood cell types over the past two decades.
Understanding hematopoiesis has improved the efficiency of generating blood cell progenitors from hPSCs.

Purpose of the Study:

To review recent strategies for generating hematopoietic stem cells and mature blood cells from hPSCs.
To evaluate the efficiency and mechanisms of these differentiation approaches.
To discuss challenges and potential solutions for clinical translation of hPSC-derived blood cells.

Main Methods:

Review of literature on hPSC differentiation protocols for blood cell generation.
Analysis of efficiency, scalability, and functional maturity of hPSC-derived blood cells.
Discussion of challenges in large-scale production and clinical application.

Main Results:

Various differentiation strategies can produce diverse blood cell types from hPSCs.
Higher efficiencies in generating hematopoietic progenitors and precursors have been achieved.
Generating large-scale, mature, functional blood cells from hPSCs for clinical use is still a significant hurdle.

Conclusions:

Efficient, simple, and defined methodologies are emerging for hPSC-derived blood cells.
Addressing challenges in scale-up and functional maturity is crucial for clinical translation.
Developing good manufacturing practice standards will facilitate the clinical application of these cells.