Related Experiment Video
Updated: Jul 17, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Co-encapsulating Cofactor and Enzymes in Hydrogen-Bonded Organic Frameworks for Multienzyme Cascade Reactions with
1Department of Biochemical Engineering, School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology, Ministry of Education), Tianjin University, Tianjin, 300350, P. R. China.
Abstract:
Use of hydrogen-bonded organic frameworks (HOFs) for enzyme immobilization faces challenges in the improvement of enzyme activity recovery and the assembly of cofactor-dependent multienzyme systems. Herein, we report a polyelectrolyte-assisted encapsulation approach (PAEA) that enables two cascades with four oxidoreductases and two nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) cofactors co-encapsulated in BioHOF-1 with excellent cargo loading and over 100 % cascade activity. The key role of the polyelectrolyte is to coat enzymes and tether NAD(P)H, thus interacting with HOF monomers in place of enzymes, avoiding the destruction of enzymes by HOF monomers. The versatility and efficiency of PAEA are further illustrated by an HOF-101-based bio-nanoreactor. Moreover, the immobilization by PAEA makes enzymes and NAD(P)H display excellent stability and recyclability. This study has demonstrated a facile and versatile PAEA for fabricating cofactor-dependent multienzyme cascade nanoreactors with HOFs.
Related Concept Videos
Cofactors and Coenzymes
Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
Introduction to Mechanisms of Enzyme Catalysis
Enzymes
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Introduction to Enzymes
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that...
Catalytically Perfect Enzymes
Most enzymes...
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...

