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Related Concept Videos

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Two-Dimensional Microscopy in Microbiology01:29

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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Overview of Electron Microscopy01:25

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Related Experiment Video

Updated: Jul 17, 2025

Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion
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MicroMagnify: A Multiplexed Expansion Microscopy Method for Pathogens and Infected Tissues.

Zhangyu Cheng1, Caroline Stefani2, Thomas Skillman3

  • 1Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 2, 2023
PubMed
Summary
This summary is machine-generated.

MicroMagnify (µMagnify) enhances nanoscale imaging of pathogens and infected tissues by enabling high-plex fluorescence imaging with up to eightfold expansion. This novel method aids in understanding host-microbe interactions and developing new diagnostic strategies for infectious diseases.

Keywords:
expansion microscopyinfected tissuemicrobiologymultiplexing

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Area of Science:

  • Microbiology
  • Optical Imaging
  • Pathogen Research

Background:

  • Super-resolution microscopy is vital for microbial studies but limited for pathogens and infected tissues.
  • Existing methods struggle with detailed nanoscale imaging of complex biological samples.

Purpose of the Study:

  • To develop a nanoscale multiplexed imaging method for pathogens and infected tissues.
  • To improve the visualization of microbial structures and host-pathogen interactions.

Main Methods:

  • MicroMagnify (µMagnify) utilizes expansion microscopy with a universal biomolecular anchor.
  • Combines heat denaturation and enzyme cocktails for robust cell wall digestion and tissue expansion.
  • Enables high-plex fluorescence imaging with nanoscale precision.

Main Results:

  • Achieved up to eightfold expansion on diverse samples: bacterial/fungal biofilms, infected cells, and human/mouse tissues.
  • µMagnify retains biomolecules for detailed imaging without distortion.
  • Developed an associated virtual reality tool for immersive 3D image visualization and collaboration.

Conclusions:

  • µMagnify is a powerful imaging platform for studying microbe-host interactions.
  • This method facilitates the development of novel diagnostic strategies for infectious diseases.
  • Enables advanced research in microbiology and pathology.