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Related Experiment Video

Updated: Jul 17, 2025

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
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Superior detection rate of plasma cell FISH using FACS-FISH.

Marie-France Gagnon1, Sally M Midthun1, James A Fangel1

  • 1Department of Laboratory Medicine and Pathology, Division of Laboratory Genetics, Mayo Clinic, Rochester, MN, US.

American Journal of Clinical Pathology
|September 2, 2023
PubMed
Summary

Fluorescence-activated cell sorting FISH (FACS-FISH) offers improved diagnostic yield for plasma cell neoplasms (PCNs) compared to cytoplasmic immunoglobulin FISH (cIg-FISH). FACS-FISH enhances detection rates and reduces workflow requirements in clinical settings.

Keywords:
FACS-FISHFISH testingcIg-FISHcytoplasmic immunoglobulin FISHfluorescence-activated cell sorting FISHmultiple myelomaplasma cell neoplasm

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Area of Science:

  • Hematology
  • Cytogenetics
  • Molecular Diagnostics

Background:

  • Plasma cell neoplasms (PCNs) diagnosis relies on FISH, necessitating efficient plasma cell (PC) identification or purification.
  • Current methods like cytoplasmic immunoglobulin FISH (cIg-FISH) have limitations in optimizing results.

Purpose of the Study:

  • To compare the diagnostic efficacy of cIg-FISH and fluorescence-activated cell sorting FISH (FACS-FISH) for PCNs in a clinical setting.
  • To evaluate the impact of FACS-FISH on detection rates, workflow, and cost-effectiveness.

Main Methods:

  • Analysis of 14,855 FISH tests for suspected PCNs from 2019-2022.
  • Comparison of cIg-FISH (n=6917) and FACS-FISH (n=7938) results.
  • Evaluation of specific genetic abnormalities and PC yield.

Main Results:

  • FACS-FISH demonstrated a higher detection rate of abnormalities (54% vs. 50%, P < .001) compared to cIg-FISH.
  • FACS-FISH significantly improved detection of IGH::CCND1, IGH::MAF, IGH::MAFB, other IGH rearrangements, and 1q gains/amplifications.
  • Insufficient PC yield for FISH was reduced from 22% (cIg-FISH) to 3% (FACS-FISH), with ploidy status established in 91% of cases.

Conclusions:

  • FACS-FISH is a superior PC purification strategy for PCNs, significantly enhancing diagnostic yield.
  • FACS-FISH streamlines workflow, reduces analysis time, and lowers operating costs compared to cIg-FISH.