Variability in the PRDM9 gene in Sindhi cattle.
Victoria Camilla Parente Rocha1, Jackeline Santos Alves1, Raphael Bermal Costa1
1Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia, (UFBA), Salvador, BA, Brasil.
Molecular Biology Reports
|September 2, 2023
Summary
Genetic diversity in Sindhi cattle can be enhanced by understanding PRDM9 gene alleles. Characterizing these alleles aids in selective mating strategies to boost genetic variability and improve the breed's resilience.
Area of Science:
- * Animal Genetics and Genomics
- * Livestock Breeding and Genetics
Background:
- * Sindhi cattle, a tropical breed, face low population numbers and high inbreeding in Brazil.
- * Increasing genetic diversity is crucial for the breed's sustainability.
- * The PRDM9 gene influences meiotic recombination, potentially increasing genetic variability.
Purpose of the Study:
- * To characterize the alleles of the PRDM9 gene in Sindhi cattle.
- * To identify potential strategies for enhancing genetic diversity within the breed.
Main Methods:
- * Amplification of the zinc finger domain region of the PRDM9 gene via PCR.
- * Genotyping and sequencing of the amplified region in 50 Sindhi cattle.
- * Identification of PRDM9 alleles and genotypes.
Main Results:
- * Three distinct PRDM9 alleles were identified: A-cattle1, B-cattle14, and C-cattle19.
- * Six genotypes were observed: AA, BB, CC, AB, AC, and BC.
Conclusions:
- * The identified allele variation in the PRDM9 gene offers opportunities for managing genetic diversity.
- * Selective mating based on PRDM9 genotypes/alleles can promote intralocus variability and enhance recombination rates.
- * This approach can contribute to increasing overall genetic variability in the Sindhi cattle population.
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