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Updated: Jul 17, 2025
Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Published on: September 2, 2019
Detection of genetic divergence among putative ethyl methane sulfonate mutants of super basmati using microsatellite
Kiran Hameed1, Madiha Habib2, Faisal Saeed Awan3
1Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Jail Road, Faisalabad, 38040, Pakistan.
Background:
Seeds of super basmati were mutagenized with different ethyl methane sulphonate (EMS) doses for creating genetic variability.
Methods And Results:
A total of 48 randomly selected putative EMS mutants of super basmati were analyzed to dissect the genetic diversity by using 25 SSR primers located on twelve chromosomes of rice. SSRs analysis revealed that wide-range of genetic diversity is present among mutants of super basmati. A sum of 91 alleles were identified, out of these, 82 alleles were polymorphic and the rest of nine alleles were monomorphic in nature. The range of allele number was 2-10 with mean of 3.64 alleles/locus. The value of polymorphic information content was range between 0.039 (RM5) and 0.878 (RM44) with mean of 0.439 for each locus. A number of polymorphic markers showed unique bands of various sizes ranges from 75 to 1000 bp, during genetic dissection of mutant population. Dendrogram divided whole mutant population into four major groups. Phylogenic analyses revealed that 40-96%genetic similarity is present among individuals of mutant population.
Conclusion:
It is concluded that EMS induced genetic variability and SSRs markers (RM44, RM154, RM1, RM252, RM334, RM487, RM110 and RM257) could be handy for the selection of rice mutants as parents for functional genomic and molecular breeding program.
