Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translational Regulation01:29

Translational Regulation

42
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
42
RNA Stability01:53

RNA Stability

33.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.6K
Types of RNA01:23

Types of RNA

63.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.9K
Master Transcription Regulators02:23

Master Transcription Regulators

6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

943
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
943
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

5.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparing the Update of the Reporting Items for Practice Guidelines in HealThcare (RIGHT) Statement: Analysis of Comments and Suggestions From the Scientific Literature.

Journal of evidence-based medicine·2026
Same author

Interpretable machine learning model for predicting operative difficulty in robotic total mesorectal excision for mid-low rectal cancer.

Journal of robotic surgery·2026
Same author

Roles and mechanisms of Pueraria lobata radix in metabolic dysfunction-associated steatotic liver disease.

Journal of ethnopharmacology·2026
Same author

The impact of psycho-physical training in esports on reaction time and decision-making in competitive athletes.

BMC psychology·2026
Same author

Association of cumulative exposure and dynamic change patterns of metabolic syndrome score with cardiometabolic multimorbidity progression among middle-aged and older Chinese adults: a longitudinal analysis based on CHARLS.

Cardiovascular diabetology. Endocrinology reports·2026
Same author

Nontargeted screening of carbonyl-containing hormonal residues in meat products for food safety by using intelligent high-resolution mass spectrometry-based workflow.

Food chemistry·2026

Related Experiment Video

Updated: Jul 17, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K

RNA m6A methylation regulators in sepsis.

Lin Zhu1, Hairong Zhang2, Xiaoyu Zhang1

  • 1School of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, People's Republic of China.

Molecular and Cellular Biochemistry
|September 2, 2023
PubMed
Summary

N6-methyladenosine (m6A) modifications play a key role in sepsis development. Understanding m6A regulators offers potential for new sepsis diagnostics and treatments.

Keywords:
SIRSSepsisSepsis shockm6A modification

More Related Videos

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.4K
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.3K

Related Experiment Videos

Last Updated: Jul 17, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.4K
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.3K

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Immunology

Background:

  • N6-methyladenosine (m6A) is a crucial RNA modification involved in various biological processes.
  • m6A modification is dynamically regulated by "Writers", "Erasers", and "Readers" proteins.
  • Sepsis pathogenesis is increasingly linked to dysregulated m6A modifications.

Purpose of the Study:

  • To review current research on the role of m6A modifications and their regulators in sepsis.
  • To explore the therapeutic potential of targeting m6A pathways for sepsis treatment.
  • To discuss the diagnostic and prognostic value of m6A in sepsis.

Main Methods:

  • Literature review of recent studies on m6A in sepsis.
  • Analysis of the functions of m6A "Writers" (METTL3/14/WTAP), "Erasers" (FTO, ALKBH5), and "Readers" (YTHDC1/2, YTHDF1/2/3).
  • Synthesis of findings regarding m6A's impact on sepsis development and clinical outcomes.

Main Results:

  • m6A modification and its regulators are significantly involved in sepsis progression.
  • Specific m6A "Writers", "Erasers", and "Readers" influence sepsis-related immune responses and organ damage.
  • Evidence suggests m6A modulators can impact sepsis severity and survival rates.

Conclusions:

  • m6A modification is a critical factor in sepsis.
  • Targeting m6A regulators presents a promising avenue for novel sepsis therapies.
  • m6A-based biomarkers may aid in sepsis diagnosis and prognosis.