Related Experiment Video
Updated: Jul 17, 2025
Pancreatic Tissue Dissection to Isolate Viable Single Cells
Published on: May 26, 2023
Protocol for identifying immune checkpoint on circulating tumor cells of human pancreatic ductal adenocarcinoma by
Xiaowei Liu1, Jinen Song1, Xinyu Liu1
1Institute for Breast Health Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
Abstract:
Circulating tumor cells (CTCs) are regarded as the "seeds" of tumor metastasis. Identifying immune checkpoints on CTCs is essential for establishing efficient immunotherapies to prevent tumor metastasis. Here, we present a protocol for isolating CTCs and obtaining single-cell suspensions from pancreatic ductal adenocarcinoma liver metastatic patients. We describe steps for biospecimen acquisition, CTC isolation, and tissue dissociation. We then detail procedures for performing single-cell RNA-seq, annotating cell types, and identifying immune checkpoints on CTCs. For complete details on the use and execution of this protocol, please refer to Liu et al. (2023).1.
