[Analysis of errors in determining the aspiration dosage in aerosol immunization and in the experimental study of...

The present work deals with the factors affecting the accuracy of the dosage of vaccinal preparations in the aerosol immunization of animals in a dynamic chamber. The method for analysis of errors in the determination of the aspiration dose is proposed. The method is based on mathematical models describing the main parameters of aerosols and the respiratory factors of the animals, the errors of the techniques and equipment used for immunization. The errors in the determination of the dose have been shown to decrease the reliability of results obtained in the evaluation of respiratory ED50 of vaccinal preparations and to impose limits on the required number of experimental animals.