Related Experiment Video

Updated: Jul 17, 2025

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
07:48

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

Published on: December 26, 2016

11.4K

hsa_circ_0003596, as a novel oncogene, regulates the malignant behavior of renal cell carcinoma by modulating

QingZhi Xie1, FuQiang Qin1, LiHui Luo2

  • 1Department of Urology Surgery, The First Affiliated Hospital of Shaoyang University, Shaoyang, 422000, Hunan, China.

European Journal of Medical Research
|September 2, 2023
PubMed
Abstract

Insights

Circular RNA circCOL5A1 promotes renal cell carcinoma (RCC) by enhancing glycolysis. Silencing circCOL5A1 inhibits tumor growth and malignancy, offering a potential therapeutic target for RCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Renal cell carcinoma (RCC) is a significant health concern.
  • Understanding the molecular mechanisms driving RCC progression is crucial for developing effective treatments.
  • Dysregulation of circular RNAs and metabolic pathways like glycolysis are implicated in various cancers.

Purpose of the Study:

  • To investigate the role of hsa_circ_0003596 (circCOL5A1) in renal cell carcinoma (RCC).
  • To elucidate the involvement of glycolysis-focused mechanisms in circCOL5A1-mediated RCC progression.
  • To explore the circCOL5A1/miR-370-5p/PRKCSH regulatory axis in RCC.

Main Methods:

  • Quantification of circCOL5A1, miR-370-5p, and PRKCSH expression in RCC tissues and cell lines using RT-qPCR and Western blot.
  • Functional assays including colony formation, EdU, Transwell, and flow cytometry to assess cell proliferation, invasion, migration, and apoptosis.
  • Glycolysis evaluation through glucose consumption, lactate, ATP production, and key glycolytic enzyme expression (HK2, PKM2).
  • In vivo studies involving circCOL5A1 knockdown in animal models.
  • Luciferase reporter and RIP assays to confirm the ceRNA network.

Main Results:

  • circCOL5A1 and PRKCSH were upregulated, while miR-370-5p was downregulated in RCC.
  • circCOL5A1 knockdown suppressed RCC cell proliferation, invasion, migration, and glycolysis, while enhancing apoptosis.
  • circCOL5A1 acts as a sponge for miR-370-5p, and miR-370-5p targets PRKCSH.
  • Overexpression of miR-370-5p counteracted the pro-tumor effects of circCOL5A1, and PRKCSH overexpression reversed the anti-tumor effects of circCOL5A1 silencing.
  • Silencing circCOL5A1 inhibited tumor growth and glycolysis in vivo.

Conclusions:

  • circCOL5A1 promotes the malignant behavior of RCC by modulating glycolysis.
  • The circCOL5A1/miR-370-5p/PRKCSH axis plays a critical role in RCC pathogenesis.
  • circCOL5A1 represents a potential therapeutic target for renal cell carcinoma.
Keywords:
GlycolysisRenal cell carcinomahsa_circ_0003596miR-370-5p; PRKCSH

More Related Videos

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

18.0K
Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.6K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.0K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.6K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K