Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

53
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
53
Experimental RNAi02:15

Experimental RNAi

6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mast-cell derived nerve growth factor drives ILC2 pro-tumoral functions in bladder cancer.

Nature communications·2026
Same author

Single-amino acid variants in target epitopes can confer resistance to antibody-based therapies.

Science translational medicine·2025
Same author

Basic Concepts and Indications of CAR T Cells.

Hamostaseologie·2025
Same author

Selective haematological cancer eradication with preserved haematopoiesis.

Nature·2024
Same author

Melanoma Clonal Heterogeneity Leads to Secondary Resistance after Adoptive Cell Therapy with Tumor-Infiltrating Lymphocytes.

Cancer immunology research·2024
Same author

TGF-β specifies T<sub>FH</sub> versus T<sub>H</sub>17 cell fates in murine CD4<sup>+</sup> T cells through c-Maf.

Science immunology·2024

Related Experiment Video

Updated: Jul 17, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

5.7K

Leveraging microRNAs for cellular therapy.

Marko Hasiuk1, Marianne Dölz1, Romina Marone1

  • 1Department of Biomedicine, Basel University Hospital and University of Basel, Hebelstrasse 20, CH-4031 Basel, Switzerland; Transplantation Immunology & Nephrology, Basel University Hospital, Petersgraben 4, CH-4031 Basel, Switzerland.

Immunology Letters
|September 3, 2023
PubMed
Summary
This summary is machine-generated.

Engineered cellular therapies can be enhanced using microRNAs (miRNAs), a key layer of gene regulation. This approach offers a promising avenue for developing more sophisticated and effective cell-based treatments.

Keywords:
Cell therapyNon-coding RNASynthetic biologyT cellmiR-17∼92microRNA

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K
In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

7.4K

Related Experiment Videos

Last Updated: Jul 17, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

5.7K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K
In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

7.4K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunotherapy

Background:

  • Cellular therapies have evolved significantly since the early 1900s, from simple blood transfusions to complex chimeric antigen receptor (CAR) T cells.
  • Modern cell engineering leverages advances in cell biology and molecular genetics to create programmable therapeutic cells.
  • While protein engineering dominates cell therapy development, non-coding RNAs, particularly microRNAs (miRNAs), play a crucial role in posttranscriptional gene regulation.

Purpose of the Study:

  • To highlight the potential of incorporating microRNAs (miRNAs) into engineered cellular therapies.
  • To demonstrate how miRNAs can be utilized to enhance the functionality and precision of therapeutic cells.
  • To underscore the importance of exploring non-coding RNA-based strategies in cell engineering.

Main Methods:

  • Reviewing existing literature on microRNA function and cell engineering techniques.
  • Identifying examples where miRNAs have been successfully integrated into engineered cell-based therapeutic strategies.
  • Analyzing the impact of miRNA incorporation on cellular behavior and therapeutic outcomes.

Main Results:

  • MicroRNAs offer a powerful mechanism for fine-tuning gene expression in engineered cells.
  • Successful integration of miRNAs can lead to improved control over cellular responses and therapeutic efficacy.
  • Examples demonstrate the feasibility and benefits of using miRNAs in advanced cellular therapies.

Conclusions:

  • MicroRNAs represent a valuable, yet underutilized, component for enhancing engineered cellular therapies.
  • Future cell engineering efforts should increasingly consider the strategic use of miRNAs for precise therapeutic control.
  • Harnessing miRNA-mediated gene regulation can drive innovation in the development of next-generation cell-based medicines.