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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Drug Discovery: Overview01:26

Drug Discovery: Overview

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Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
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Related Experiment Video

Updated: Jul 17, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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Affinity selection mass spectrometry speeding drug discovery.

Renaud Prudent1, Hugues Lemoine1, Jarrod Walsh2

  • 1Edelris, Bioparc, Bioserra 1 Building, Lyon, France.

Drug Discovery Today
|September 3, 2023
PubMed
Summary

Affinity selection mass spectrometry (AS-MS) is emerging as a powerful tool for drug discovery hit identification. Its synergy with DNA encoded libraries offers new avenues for identifying small molecule binders for challenging targets, including RNA.

Keywords:
affinity selection mass spectrometrydrug discoveryhit identification

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Area of Science:

  • Biochemistry and Medicinal Chemistry
  • Analytical Chemistry and Mass Spectrometry
  • Drug Discovery and Development

Background:

  • Affinity selection mass spectrometry (AS-MS) has become increasingly significant in modern drug discovery pipelines.
  • The technology offers a robust method for identifying small molecules that bind to specific biological targets.
  • Its potential for integration with other cutting-edge technologies, like DNA encoded libraries, is a key area of interest.

Purpose of the Study:

  • To review the rise of AS-MS as a paradigm shift in hit identification strategies.
  • To explore the synergistic potential between AS-MS and DNA encoded library technology.
  • To discuss recent advancements in applying AS-MS to challenging drug targets and novel research areas like RNA targeting.

Main Methods:

  • Review of recent literature and case studies showcasing AS-MS applications.
  • Analysis of AS-MS synergy with DNA encoded libraries for hit identification.
  • Discussion of strategic implementation based on experiences from Contract Research Organizations (CROs) and large pharmaceutical companies.

Main Results:

  • AS-MS has demonstrated significant success in identifying hits against difficult targets.
  • The integration of AS-MS with DNA encoded libraries enhances the efficiency and scope of hit discovery.
  • The technology's versatility is highlighted through its application in diverse research areas, including RNA-small molecule interactions.

Conclusions:

  • AS-MS is a versatile and powerful technology for hit identification in drug discovery.
  • Its combination with DNA encoded libraries presents a promising strategy for tackling challenging targets.
  • Future research directions include expanding AS-MS applications to novel areas such as RNA targeting.