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Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
Published on: June 9, 2023
LINC-PINT suppresses breast cancer cell proliferation and migration via MEIS2/PPP3CC/NF-κB pathway by sponging
1Department of Pathology, Henan Provincial People's Hospital, Jinshui District, Zhengzhou, Henan, China.
Background:
Breast cancer (BCa) is the most frequent malignant tumor in women. Long non-coding RNAs (lncRNAs) have been acknowledged to exert critical regulating functions in various cancers. Long intergenic non-protein coding RNA, p53 induced transcript (LINC-PINT) has been reported to be a chemosensitizer and a tumor suppressor in BCa. However, its downstream molecular mechanism contributing to its tumor-suppressing role remains to be explored in BCa.
Methods:
LINC-PINT expression in BCa tissues and cells was measured using quantitative real-time polymerase chain reaction (RT-qPCR). The proliferation of transfected BCa cells was examined by counting kit-8 (CCK-8) and EdU assay. The migrating ability of indicate BCa cells was assessed by wound healing assays. Bioinformatics analysis and mechanism experiments such as RNA immunoprecipitation (RIP), RNA pull down assay, and luciferase reporter assay, were applied to demonstrate the downstream targets of LINC-PINT.
Results:
LINC-PINT was downregulated in BCa tissues and cell lines. Overexpression of LINC-PINT suppressed BCa cell proliferation and migration. LINC-PINT could interact with miR-576-5p to upregulate Meis homeobox 2 (MEIS2) that positively regulated protein phosphatase 3 catalytic subunit gamma (PPP3CC) by inactivating the nuclear factor-κB (NF-κB) pathway.
Conclusions:
These findings elucidated the anti-tumor role of LINC-PINT in BCa via the miR-576-5p/MEIS2/PPP3CC/NF-κB axis, which suggested that LINC-PINT might serve as a potential therapeutic target for BCa.
Insights
Long intergenic non-protein coding RNA, p53 induced transcript (LINC-PINT) acts as a tumor suppressor in breast cancer (BCa). Its downregulation promotes BCa progression by affecting the miR-576-5p/MEIS2/PPP3CC/NF-κB pathway.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Breast cancer (BCa) is a prevalent malignancy in women.
- Long non-coding RNAs (lncRNAs) play crucial roles in cancer regulation.
- Long intergenic non-protein coding RNA, p53 induced transcript (LINC-PINT) shows tumor-suppressive and chemosensitizing effects in BCa, but its mechanism is unclear.
Purpose of the Study:
- To investigate the downstream molecular mechanism of LINC-PINT's tumor-suppressing role in breast cancer.
- To elucidate the regulatory pathway involving LINC-PINT in BCa progression.
Main Methods:
- Quantitative real-time polymerase chain reaction (RT-qPCR) for LINC-PINT expression.
- Cell proliferation assays (CCK-8, EdU) and migration assays (wound healing).
- Bioinformatics, RNA immunoprecipitation (RIP), RNA pull down, and luciferase reporter assays to identify downstream targets.
Main Results:
- LINC-PINT expression was significantly downregulated in BCa tissues and cell lines.
- Overexpression of LINC-PINT inhibited BCa cell proliferation and migration.
- LINC-PINT interacts with miR-576-5p, upregulating MEIS2, which activates PPP3CC by inhibiting the NF-κB pathway.
Conclusions:
- LINC-PINT exerts anti-tumor effects in BCa through the miR-576-5p/MEIS2/PPP3CC/NF-κB signaling axis.
- LINC-PINT represents a potential therapeutic target for breast cancer treatment.
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