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Application of CRISPR-Cas system in the diagnosis and therapy of ESKAPE infections

Yizheng Qian1,2, Dapeng Zhou1,2,3, Min Li1,2

  • 1Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China.

Frontiers in Cellular and Infection Microbiology
|September 4, 2023
PubMed

Insights

Antimicrobial resistance is a major threat, with ESKAPE pathogens causing difficult-to-treat infections. The CRISPR-Cas system shows promise for developing new antimicrobial strategies against these superbugs, though clinical applications are still distant.

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) are a critical global health concern due to widespread antimicrobial resistance.
  • These opportunistic pathogens are a leading cause of nosocomial infections, with many strains resistant to current antibiotic treatments.
  • The rise of multidrug-resistant bacteria necessitates novel therapeutic approaches to combat infections and alleviate patient suffering.

Purpose of the Study:

  • To review the current applications of the CRISPR-Cas system in studying ESKAPE pathogens.
  • To explore the potential of CRISPR-based technologies as innovative antimicrobial strategies.
  • To provide insights for developing new drugs and addressing challenges posed by multidrug-resistant bacteria in the post-antibiotic era.

Main Methods:

  • Literature review of CRISPR-Cas system applications against ESKAPE pathogens.
  • Analysis of research focusing on the specificity and potential of CRISPR-based antimicrobials.
  • Evaluation of the current stage of research concerning clinical applicability.

Main Results:

  • The CRISPR-Cas system offers high specificity for targeting pathogens.
  • Existing research demonstrates the potential of CRISPR-Cas for studying ESKAPE pathogens.
  • Despite promising research, direct CRISPR-system-based anti-infective treatments are not yet available.

Conclusions:

  • CRISPR-Cas technology holds significant potential for developing novel antimicrobial therapies against ESKAPE pathogens.
  • Further research is required to translate CRISPR-based strategies from laboratory studies to clinical applications.
  • This review provides a reference for future drug development and strategies to combat multidrug-resistant bacteria.
Keywords:
CRISPR-CasESKAPEdiagnosisinfectionpathogentherapy

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