Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

18.8K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
18.8K
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

197
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
197

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell wall dynamics and biomechanics of root hair morphogenesis.

Journal of experimental botany·2026
Same author

The carbohydrate-binding module of TrCel7A aids in navigating hemicellulose barriers in plant cell walls.

The Journal of biological chemistry·2026
Same author

Mechanical constraint causes lower turgor, thicker walls, and faster growth in Arabidopsis root hairs.

Plant physiology·2026
Same author

Context-dependent effects of SOM neurons to enhance the responses of corticocollicular neurons to repetitive sounds.

iScience·2026
Same author

XTH genes impact growth habit determination of twining common bean vine revealed by brassinosteroid treatment.

The New phytologist·2026
Same author

The epidermis coordinates multi-scale symmetry breaking in chiral root growth.

Nature communications·2025

Related Experiment Video

Updated: Jul 17, 2025

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
11:42

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis

Published on: November 20, 2013

12.1K

Measuring calcium content in plants using NEXAFS spectroscopy.

Sintu Rongpipi1, William J Barnes2, Oskar Siemianowski2

  • 1Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, United States.

Frontiers in Plant Science
|September 4, 2023
PubMed
Summary

Near-edge X-ray absorption fine structure (NEXAFS) spectroscopy offers a new method to measure plant calcium levels. This technique quantifies calcium in plant tissues without chemical extraction, aiding plant biology and industry applications.

Keywords:
Arabidopsis hypocotylfluorescence yield NEXAFSinductively coupled plasma mass spectrometryonion epidermisprimary cell walltransmission NEXAFS

More Related Videos

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar
07:19

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar

Published on: August 6, 2018

19.9K
Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
10:12

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

Published on: September 2, 2014

12.1K

Related Experiment Videos

Last Updated: Jul 17, 2025

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
11:42

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis

Published on: November 20, 2013

12.1K
Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar
07:19

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar

Published on: August 6, 2018

19.9K
Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
10:12

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

Published on: September 2, 2014

12.1K

Area of Science:

  • Plant Biology
  • Spectroscopy
  • Biogeochemistry

Background:

  • Calcium is vital for plant growth, cell structure, and signaling pathways.
  • Accurate calcium measurement is crucial for plant science and related industries.
  • Existing methods like AAS and ICP-MS have limitations in sample size, extraction, and spatial resolution.

Purpose of the Study:

  • To develop and validate a novel method for quantifying calcium in plant samples.
  • To assess the utility of near-edge X-ray absorption fine structure (NEXAFS) spectroscopy for plant calcium analysis.
  • To overcome the limitations of traditional calcium measurement techniques in plants.

Main Methods:

  • Utilized near-edge X-ray absorption fine structure (NEXAFS) spectroscopy at calcium L- and K-edges.
  • Measured the calcium to carbon mass ratio with spatial resolution in plant samples.
  • Validated the NEXAFS approach using onion epidermal peels and inductively coupled plasma-mass spectrometry (ICP-MS).

Main Results:

  • NEXAFS spectroscopy successfully quantified calcium mass fraction in plant samples without chemical extraction.
  • The integrated absorbance at the calcium L-edge and edge jump at the calcium K-edge correlated with calcium content.
  • Calcium mass ratio was accurately estimated in *Arabidopsis thaliana* hypocotyls.

Conclusions:

  • NEXAFS spectroscopy provides a powerful, non-destructive method for quantifying calcium levels in plants.
  • This technique enhances the study of plant physiology and the development of plant-based materials.
  • NEXAFS offers improved spatial resolution and reduced sample requirements compared to traditional methods.