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Regulated Protein Degradation

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It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
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Related Experiment Video

Updated: Jul 17, 2025

Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

Published on: February 4, 2018

Evaluating single-cell variability in proteasomal decay.

Sukanya Das¹, Abhyudai Singh², Premal Shah¹

¹Department of Genetics, Rutgers University.

Biorxiv : the Preprint Server for Biology

|September 4, 2023

Summary

This summary is machine-generated.

Gene expression noise arises from protein decay variability. This study reveals significant cell-to-cell differences in protein degradation rates, impacting gene expression stochasticity.

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Assays for the Degradation of Misfolded Proteins in Cells

Assays for the Degradation of Misfolded Proteins in Cells

Published on: August 28, 2016

Area of Science:

Molecular Biology
Cell Biology
Biophysics

Background:

Gene expression exhibits inherent variability (noise) in protein and mRNA levels, even in identical cells.
This noise is often attributed to transcription and translation, overlooking protein decay dynamics.
Understanding protein decay is crucial for a complete model of gene expression noise.

Conclusions:

Protein decay rates exhibit significant variability at the single-cell level.
Stochasticity in protein decay contributes to overall gene expression noise.
Accurate modeling of gene expression noise requires incorporating the complex kinetics of protein decay.